[14C] 5,5 dimethyloxazolidine-2,4-dione-2-14C (DMO) was generous gifts of Dr. H. R. Kaback (University of California, Los Angeles). Tetraphenylphosphonium [3H]-TPP came from Amersham. SuperSignal West Pico chemiluminescent substrates for detection of horseradish peroxidase came from Thermo Fisher Scientific). Antibodies directed against core translocon components, YidC and OmpF were a generous gift of Professors Ken‐ichi Nishiyama (Iwate University, Morioka, Japan), Tassos Economou (KU Leuven - University of Leuven) and Ross Dalbey (Ohio State University), George Koch (Institute of Biochemistry and Molecular Biology, Albert-Ludwigs University Freiburg) and Hiroshi Nikaido (University of California, Berkeley), respectively. Molecular biology grade chemicals were purchased from Sigma.
Molecular biology grade chemicals
Manufactured by Merck Group
Molecular biology grade chemicals are high-purity reagents designed for use in molecular biology applications. They are manufactured to meet stringent quality standards and are suitable for a variety of laboratory procedures, including DNA and RNA manipulation, protein analysis, and cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor antibodies, by Jackson ImmunoResearch (1 mentions)
Ir dye labeled reagents, by LI COR (1 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions)
Aspirin, by Merck Group (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
2 protocols using molecular biology grade chemicals
1
Radiolabeled Compound Characterization and Antibody Sourcing
2
Cell Culture Materials Procurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture materials (DMEM/F-12, L-glutamine, Hanks’ balanced salt solution, 0.05%trypsin, and antibiotic/antimycotic) were purchased from ThermoFisher. Fetal bovine serum (FBS) was obtained from Atlas Biologicals. Aspirin and all molecular biology-grade chemicals were obtained from Sigma-Aldrich Inc. Sources of primary antibodies used in this study are mentioned in Table 1 . Alexa-fluor antibodies used in immunostaining were obtained from Jackson Immuno Research and IR dye-labeled reagents used for immunoblotting were from Li-Cor Biosciences.
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