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Total egfr

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Total EGFR is a lab equipment product that measures the total expression of the Epidermal Growth Factor Receptor (EGFR) protein in biological samples. It provides quantitative information about the overall EGFR levels, without distinguishing between different EGFR isoforms or activation states.

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Lab products found in correlation

Bcl 2, by Agilent Technologies (2 mentions) Mcl 1, by BD (2 mentions) P erk1 2, by Cell Signaling Technology (2 mentions) P egfr, by Cell Signaling Technology (2 mentions) Total erk1 2, by Cell Signaling Technology (2 mentions) Coxiv, by Thermo Fisher Scientific (2 mentions) Total foxo3a, by Merck Group (2 mentions) Total aurora a, by Cell Signaling Technology (2 mentions) Total aurora b, by Cell Signaling Technology (2 mentions) P aurora a b c, by Cell Signaling Technology (2 mentions) P foxo3a, by Cell Signaling Technology (2 mentions) P akt, by Cell Signaling Technology (2 mentions) Total akt, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Cleaved caspase 9, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) Cytochrome c, by Santa Cruz Biotechnology (2 mentions) Bcl2 associated x (bax), by BD (2 mentions) Bcl xl, by BD (2 mentions) Phospho egfr, by Abcam (1 mentions)

3 protocols using total egfr

1

Comprehensive Western Blotting Analysis of Cell Signaling

Cited 1 time
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Western blotting was performed as previously described [41 (link)] using antibodies against: β-Actin (A5441, Sigma), cleaved caspase-3 (#9661, Cell Signaling, Danvers, MA, USA), cleaved caspase-9 (#9502, Cell Signaling), Mcl-1 (#559027, BD Biosciences, San Jose, CA, USA), Bax (#610983, BD Biosciences), Bid (#2002, Cell Signaling), Bcl-xL (#610212, BD Biosciences), Bim (#2819, Cell Signaling), Bcl-2 (#M0887, Agilent DAKO, Santa Clara, CA, USA), cytochrome c (sc-7159, Santa Cruz Biotechnology, Santa Cruz, CA, USA), COX IV (A21348, Invitrogen), PUMA [44 (link)], Bak (#06–536, EMD Millipore), Noxa (#OP180, EMD Millipore), p73 (A300–126A, Bethyl Laboratories, Montgomery, TX, USA), p-p73 (#4665, Cell Signaling), p-AKT (#4058, Cell Signaling), total AKT (#9272, Cell Signaling), p-ERK1/2 (#4376, Cell Signaling), total ERK1/2 (#9102, Cell Signaling), p-FoxO3A (#9464, Cell Signaling), total FoxO3A (07–702, EMD Millipore), p53 (sc-126, Santa Cruz), p-EGFR (#2234, Cell Signaling), total EGFR (#610016, BD Biosciences), KRAS (sc-30, Santa Cruz), p-Aurora A/B/C (#2914, Cell Signaling), total Aurora A (#4718, Cell Signaling), total Aurora B (#3094, Cell Signaling), and HA (#12CA5, Roche, Indianapolis, IN, USA).
Knickelbein K., Tong J., Chen D., Wang Y.J., Misale S., Bardelli A., Yu J, & Zhang L. (2018). Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer. Oncogene, 37(33), 4599-4610.
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2

Phospho-Receptor Tyrosine Kinase Assay

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Phospho-EGFR (Y1068) was from Abcam (ab40815). Total-EGFR was from BD Biosciences (#610017). Actin was from Sigma (#A2066). The following antibodies were from Cell Signaling: Phospho-antibodies against EGFR Y845 (#2231), HER2 Y1248 (#2247), HER2 Y1221/1222 (#2243), HER3 Y1197 (#4561), HER3 Y1289 (#4791), MEK1/2 S217/221 (#9154), AKT S473 (#4060), ERK1/2 T202/Y204 (#9101); total antibodies against DUSP4 (#5149), Bim (#2819), HER2 (#2242), HER3 (#4754), MEK1/2 (#9126), AKT (#9272), ERK1/2 (#9102).
Hutchinson K.E., Johnson D.B., Johnson A.S., Sanchez V., Kuba M., Lu P., Chen X., Kelley M.C., Wang Q., Zhao Z., Kris M., Berger M.F., Sosman J.A, & Pao W. (2015). ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma. Oncotarget, 6(26), 22348-22360.
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3

Comprehensive Western Blotting Analysis of Cell Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as previously described [41 (link)] using antibodies against: β-Actin (A5441, Sigma), cleaved caspase-3 (#9661, Cell Signaling, Danvers, MA, USA), cleaved caspase-9 (#9502, Cell Signaling), Mcl-1 (#559027, BD Biosciences, San Jose, CA, USA), Bax (#610983, BD Biosciences), Bid (#2002, Cell Signaling), Bcl-xL (#610212, BD Biosciences), Bim (#2819, Cell Signaling), Bcl-2 (#M0887, Agilent DAKO, Santa Clara, CA, USA), cytochrome c (sc-7159, Santa Cruz Biotechnology, Santa Cruz, CA, USA), COX IV (A21348, Invitrogen), PUMA [44 (link)], Bak (#06–536, EMD Millipore), Noxa (#OP180, EMD Millipore), p73 (A300–126A, Bethyl Laboratories, Montgomery, TX, USA), p-p73 (#4665, Cell Signaling), p-AKT (#4058, Cell Signaling), total AKT (#9272, Cell Signaling), p-ERK1/2 (#4376, Cell Signaling), total ERK1/2 (#9102, Cell Signaling), p-FoxO3A (#9464, Cell Signaling), total FoxO3A (07–702, EMD Millipore), p53 (sc-126, Santa Cruz), p-EGFR (#2234, Cell Signaling), total EGFR (#610016, BD Biosciences), KRAS (sc-30, Santa Cruz), p-Aurora A/B/C (#2914, Cell Signaling), total Aurora A (#4718, Cell Signaling), total Aurora B (#3094, Cell Signaling), and HA (#12CA5, Roche, Indianapolis, IN, USA).
Knickelbein K., Tong J., Chen D., Wang Y.J., Misale S., Bardelli A., Yu J, & Zhang L. (2018). Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer. Oncogene, 37(33), 4599-4610.
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