Cyproterone

The pharmaceutical compounds standards included Amoxicillin (AMO), Ampicillin (AMP), Cephalexin (CEPHA), Cefuroxime (CEFU), Cephotaxime (CEFO), Cipro oxacin (CIP), Clarithromycin (CLAR), Clindamycin (CLIN), Chloranphenicol (CHLOR), Cyproterone (CIP), Erythromycin (ERYT), Flutamide (FLUT), Spironolactone (SPIR), Sulfamethoxazole (SUL), Tamoxifen (TAM), Trimethoprim (TRI), these were purchased from Sigma Aldrich and used without a puri cation step. We used HPLC grade acetonitrile (ACN, 99% purity) and formic acid from (96% purity) TEDIA for the chromatographic method. Finally, all the experiments were carried out using ultrapure water. For the separation, detection, and quanti cation of pharmaceutical compounds selected, calibration curves were prepared with eight-point from standard solutions (100,000 µg/L) and the corresponding dilutions.

Chemicals and analytical reagents such as nitric acid and sulphuric acid were purchased from Merck (Darmstadt, Germany). Grade solvents used in HPLC and LC-MS, such as acetonitrile, methanol and isopropanol, were acquired from Sigma-Aldrich (Massachusetts, USA). Mobile phase additives, namely LC-MS grade formic acid and ammonium acetate, were acquired from Sigma-Aldrich and Merck, respectively. Analytical standards of acetaminophen, atenolol, bromazepam, caffeine, carbamazepine, cyproterone, clonazepam, clopidogrel, diclofenac, enalapril, loratadine, losartan, midazolam orphenadrine, propranolol, sildena l, atorvastatin, ranitidine, diazepam, chlorpheniramine and valsartan were acquired from Sigma-Aldrich. Cocaine and benzoylecgonine were acquired from Cerillant (Texas, USA). The other pharmaceuticals were bought in several suppliers: citalopram (Alcytam®: Torrent, Brazil), chlortalidone (Higroton®: Novartis, Swiss), rosuvastatin (Crestor®: AstraZeneca, UK) and generic paroxetine medication (Medley, Brazil).