Ham s f 12k

DOTAP (SC‐208,732) was obtained from Santa Cruz Biotechnology, USA. ATRA (R2625) and cholesterol (C8667) were purchased from Sigma‐Aldrich, USA. Foetal bovine serum (FBS), Ham's F‐12K (Kaighn's) medium, cholesterol, PBS, chloroform, and tween‐80 were from Himedia. PSN antibiotic mixture (15640055) was purchased from Invitrogen. All other chemicals used were of analytical grade. The A549 human lung cancer cell lines were obtained from NCCS, Pune, India.

Pyrazole was obtained from Sigma Aldrich, India. Ham's F-12 K (Kaighn's) Culture Medium, Trypsin, Fetal Bovine Serum (FBS), Bovine Serum Albumin (BSA), Antibiotic-actinomycotic solution were obtained from Hi-Media laboratories, India. Monoclonal antibodies (rabbit) such as MMP-2 (D8N9Y), NF-κB-p65 (D14E12) XpR, E-Cadherin (24E10), TNF-alpha, β-actin and HRP conjugated secondary antibodies (anti-rabbit) were purchased from Cell Signaling Technology (USA). Desalted & Hi-Pure Oligos include MMP-2, NF-κB-p65, E-Cadherin, TNF-α and β-actin were obtained from Eurofins Genomics (Bengaluru, India). Cytokine ELISA kits include TNF-α and IL-6 from Koma Biotech (Korea), iNOS and COX-2 from USCN Life science (China) were purchased.