2 naphthyl α d glucopyranoside

Acetone (99.5%), acetic acid (99.5–99.9%), diphenylboryloxyehtylamine (NP) (≥97.0%), ethanol (96%), ethyl acetate (99.8%), 2-isopropyl-5-methyl-phenol (thymol) (≥98.5%), methanol (99.8%), o-phosphoric acid (85%), polyethylene glycol—4000 (PEG-4000), phosphate buffer, sodium hydroxide (≥98%), sulfuric acid (96–98%), and sodium acetate buffer were from POCH (Gliwice, Poland). P-Anisaldehyde (AS) (≥98%), bovine serum albumin (BSA) (≤100%), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (≤100%), 3,4-dihydroxy-L-phenylalanine (L-DOPA) (>98.0%), α-glucosidase from Saccharomyces cerevisiae (≥10 units/mg protein), lipase from porcine pancreas (30–90 units/mg protein), 1-naphthyl acetate (≥98%), 2-naphthyl acetate (≤100%), 2-naphthyl α- D glucopyranoside ((≤100%), polyethylene glycol tert-octylphenyl ether (Triton X) (≤100%), rosavin (≥98.0%), salidroside (≥98.0%), p-tyrosol (98%), Fast Blue B salt (95%), tris(hydroxymethyl)aminomenthane hydrochloride (TRIS) buffer, and tyrosinase from mushroom (≥1000 unit/mg solid) were purchased from Sigma Aldrich (Poznań, Poland). Pure water was from the Millipore Q system (Millipore, Bedford, MA, USA). All reagents were of the analytical grade. Acetonitrile used in HPLC-UV/Vis-DAD quantification procedure was purchased from Romil (Cambridge, UK). TLC silica gel 60 F₂₅₄ (20 × 10 cm) plates were purchased from Merck, (Darmstadt, Germany).

Acetic acid, ethanol 96%, ethyl acetate, methanol, thymol, sulfuric acid 95%, sodium acetate buffer, and phosphate buffer were from P.O.Ch. (Gliwice, Poland). Acetylcholinesterase enzyme (AChE) from Electrophorus electricus, p-anisaldehyde, bovine serum albumin (BSA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), L-DOPA, Fast Blue B Salt, lipase from Porcine pancreas, 1-naphthyl acetate, 2-naphthyl acetate, 2-naphthyl α-D glucopyranoside, α-glucosidase from Saccharomyces, Natural Product—NP reagent (diphenylboryloxyethylamine), polyethylene glycol-4000, tyrosinase from mushroom and TRIS hydrochloride were purchased from Sigma Aldrich (Poznań, Poland). The MTT dye (3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide), HEPES buffer, and Triton X-100 were from Sigma Aldrich (Poznań, Poland). Agarose, Mueller-Hinton (M-H) agar, and M-H broth were purchased from Biocorp (Warsaw, Poland). The antibacterial activity was tested toward reference Gram-positive bacteria: Bacillus subtilis (ATCC 6633) was purchased from the American Type Culture Collections.
The standards of lignans were supplied by Chem-Faces Biochemical (Wuhan, China). The lignans working standards solutions were prepared in methanol.