Total mRNAs were isolated from cells using RNeasy mini kit (Qiagen, Hilden, Germany). Complementary DNAs were produced using the SuperScript® IV Reverse Transcriptase system (Thermo Fisher Scientific). qRT-PCR was carried out using Power SYBR Green master mix (Thermo Fisher Scientific). The qRT-PCR primers of H3.3 (i.e., H3-3A gene) was obtained from Qiagen (Cat. No. QT00247128). Gene expression levels were normalized to GAPDH using primers (forward: 5ʹ-GGAAGGTGAAGGTCGGAGTCA-3ʹ and reverse: 5ʹ-GTCATTGATGGCAACAATATCCACT-3ʹ).
H3 3a gene
Manufactured by Qiagen
The H3-3A gene is a DNA sequence that encodes for a histone protein, which is a key component of chromatin in eukaryotic cells. This gene plays a crucial role in the regulation of gene expression and chromatin structure, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
3 protocols using h3 3a gene
1
Quantitative Analysis of H3.3 Gene Expression
2
Quantifying H3.3 Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total mRNAs were isolated from cells using RNeasy mini kit (Qiagen, Hilden, Germany).
Complementary DNAs were produced using the SuperScript® IV Reverse Transcriptase system (Thermo Fisher Scientific). qRT-PCR was carried out using Power SYBR Green master mix (Thermo Fisher Scientific). The qRT-PCR primers of H3.3 (i.e. H3-3A gene) was obtained from Qiagen (Cat. No. QT00247128). Gene expression levels were normalized to GAPDH using primers (forward: 5'-GGAAGGTGAAGGTCGGAGTCA-3' and reverse: 5'-GTCATTGATGGCAACAATATCCACT-3').
Complementary DNAs were produced using the SuperScript® IV Reverse Transcriptase system (Thermo Fisher Scientific). qRT-PCR was carried out using Power SYBR Green master mix (Thermo Fisher Scientific). The qRT-PCR primers of H3.3 (i.e. H3-3A gene) was obtained from Qiagen (Cat. No. QT00247128). Gene expression levels were normalized to GAPDH using primers (forward: 5'-GGAAGGTGAAGGTCGGAGTCA-3' and reverse: 5'-GTCATTGATGGCAACAATATCCACT-3').
3
Quantifying H3.3 Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total mRNAs were isolated from cells using RNeasy mini kit (Qiagen, Hilden, Germany).
Complementary DNAs were produced using the SuperScript® IV Reverse Transcriptase system (Thermo Fisher Scientific). qRT-PCR was carried out using Power SYBR Green master mix (Thermo Fisher Scientific). The qRT-PCR primers of H3.3 (i.e. H3-3A gene) was obtained from Qiagen (Cat. No. QT00247128). Gene expression levels were normalized to GAPDH using primers (forward: 5'-GGAAGGTGAAGGTCGGAGTCA-3' and reverse: 5'-GTCATTGATGGCAACAATATCCACT-3').
Complementary DNAs were produced using the SuperScript® IV Reverse Transcriptase system (Thermo Fisher Scientific). qRT-PCR was carried out using Power SYBR Green master mix (Thermo Fisher Scientific). The qRT-PCR primers of H3.3 (i.e. H3-3A gene) was obtained from Qiagen (Cat. No. QT00247128). Gene expression levels were normalized to GAPDH using primers (forward: 5'-GGAAGGTGAAGGTCGGAGTCA-3' and reverse: 5'-GTCATTGATGGCAACAATATCCACT-3').
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