A silver-109 isotope of 99.7% isotopic purity
was bought from Trace Sciences International (USA).l -Histidine
andd -ribose were purchased from Sigma-Aldrich (99% purity).
Thymidine was purchased from Alfa Aesar (99% purity). Poly(propylene
glycol) (PPG, average Mn 1000 Da) was purchased from Sigma-Aldrich.
All solvents were of HPLC quality, except for water (18 MΩ cm
water produced locally). Steel targets were machined from H17 stainless
steel. Before the LDI MS and MS imaging experiments, steel targets
were cleaned through soaking in boiling solvents: toluene (3 ×
100 mL, each plate for 30 s), chloroform (3 × 100 mL, each plate
for 30 s), acetonitrile (3 × 100 mL, each plate for 30 s), and
deionized water (3 × 100 mL, each plate for 30 s). Every plate
was dried in high vacuum (ca. 0.01 mbar, 24 h). Optical photographs
were made with the use of an Olympus SZ10 microscope equipped with
an 8 MPix Olympus digital camera and also a Canon 6D camera with a
macro-type 90 mm focal length lens.
was bought from Trace Sciences International (USA).
and
Thymidine was purchased from Alfa Aesar (99% purity). Poly(propylene
glycol) (PPG, average Mn 1000 Da) was purchased from Sigma-Aldrich.
All solvents were of HPLC quality, except for water (18 MΩ cm
water produced locally). Steel targets were machined from H17 stainless
steel. Before the LDI MS and MS imaging experiments, steel targets
were cleaned through soaking in boiling solvents: toluene (3 ×
100 mL, each plate for 30 s), chloroform (3 × 100 mL, each plate
for 30 s), acetonitrile (3 × 100 mL, each plate for 30 s), and
deionized water (3 × 100 mL, each plate for 30 s). Every plate
was dried in high vacuum (ca. 0.01 mbar, 24 h). Optical photographs
were made with the use of an Olympus SZ10 microscope equipped with
an 8 MPix Olympus digital camera and also a Canon 6D camera with a
macro-type 90 mm focal length lens.