Anti klf4 sc 20691

The following antibodies were used in western blot: anti-FBXO32 (NBP1-36955, Novus, Littleton, CO, USA); anti-FBXO32 (ab168372, Abcam, Cambridge, MA, USA); anti-KLF4 (sc-20691, Santa-Cruz, Dallas, TX, USA); anti-KLF4 (ab75486, Abcam); anti-ubiquitin (3936, CST, Billerica, MA, USA); anti-HA (sc-805, Santa-Cruz); anti-GST (G7781, Sigma, St Louis, MO, USA); anti-Flag (F7425, Sigma), anti-Myc (562, MBL, Woburn, MA, USA). The following antibodies were used in immunohistochemistry and immunofluorescent double staining: anti-FBXO32 (LS-B9748, Lifespan Biosciences, Seattle, WA, USA) and anti-KLF4 (NBP2-24749, Novus). The anti-KLF4 antibody (12173, CST) and anti-FLAG M2 Affinity Gel (A2220, Sigma) were used for IP. The Glutathione Sepharose 4B (17-0756-01, GE Healthcare, Amersham, UK) was used in GST pulldown. Cycloheximide (C7698) and MG132 (C2211) were purchased from Sigma. SB203580 was obtained from Promega (Madison, WI, USA) and SB239063 was purchased from Selleckchem (Houston, TX, USA).