Aprotinin

HBSS, RPMI, 1640 Leibovitz's L-15 medium, trypsin, and FBS were obtained from Wisent Laboratories (St-Bruno, Quebec City, Canada). Ficoll was obtained from Corning (Tewksbury, MA, USA). Dextran was purchased from Fisher Scientific (Ottawa, ON, Canada). The CD14 and CD16 magnetic bead-coupled antibody and the MACS magnetic separation system were purchased from Miltenyi Biotec (Auburn, CA, USA). THP-1 and MDA-MB-231 cells were obtained from ATCC (Manassas, VA, USA). Human hypothalamus samples were provided by the Douglas-Bell Canada Brain Bank (Montréal, Québec, Canada). Protease inhibitor cocktail tablets were purchased from Roche (Laval, QC, Canada). Aprotinin, leupeptin, and 4′-[ [[methyl[[3-(4-pyridinyl) [1,1′-biphenyl]-4-yl ester carbamic acid (WWL70), as well as the primary antibodies for MAG lipase, were purchased from Cayman Chemical (Ann Arbor, MI, USA). The LYPLA2 primary antibody, the ABHD16A primary antibody, dextran and the fluorophosphonate (FP)-TAMRAprobewere from Thermo Fisher Scientific (Waltham, MA, USA) and the primary antibody for carboxylesterase (CES)1 was from R&D Systems (Minneapolis, MN, USA). PMSF, Palmostatin B, and the ECL detection kit were from EMD Millipore (Billerica, MA, USA). ML349 was a generous gift from Dr. Lawrence J. Marnett (Vanderbilt University, Nashville, TN, USA).