Rat anti c myc jac6

Thin smears of P. falciparum schizont stage cultures were prepared on glass slides and allowed to air-dry. The smears were fixed in 4% paraformaldehyde, 0.01% glutaraldehyde/phosphate-buffered saline (PBS) for 1 h at room temperature. Fixed smears were then permeabilized with 0.1% Triton X-100 for 10 min at room temperature and blocked with 3% BSA/PBS for 1 h at room temperature. Staining was performed with primary rat anti-c-Myc (JAC6, Abcam) diluted 1:200 and primary rabbit anti-GAP45 (43 (link)) diluted 1:500, followed by secondary Alexa-Fluor-conjugated antibodies (Life Technologies, Inc., 1:500 dilution). All antibody dilutions were performed in 1% BSA/PBS. Confocal images were acquired using a Zeiss LSM 510 laser-scanning confocal microscope.

Antibodies were used at the following dilutions and obtained from the following sources: mouse monoclonal anti-Stx1A (1:1,000), rabbit polyclonal anti-Stx3 (1:250), and rabbit polyclonal anti-Stx4 (1:250; Synaptic Systems, Göttingen, Germany), mouse anti-NgCAM 8D9, which recognizes an extracellular NgCAM epitope (1:5 of hybridoma supernatant; Developmental Studies Hybridoma Bank, Iowa City, IA), mouse anti-c-myc 9E10 (1:500; Santa Cruz Biotechnology, Santa Cruz, CA), rat anti-c-myc JAC6 (1:1,000) and rabbit anti-GFP (1:2,000; Abcam, Cambridge, MA), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:500; Chemicon, Billerica, MA), mouse anti-MAP2 (1:500; Sigma-Aldrich, St. Louis, MO), and mouse anti-Tau (Tau-5, 1:1,000; Invitrogen, Grand Island, NY). Alexa Fluor-conjugated secondary antibodies that were highly cross absorbed against multiple species (1:300), and Alexa 488-conjugated rabbit polyclonal anti-GFP (1:1,000) were from Molecular Probes (Invitrogen). Cy3-conjugated secondary antibodies (1:300) were from Jackson ImmunoResearch Laboratories (West Grove, PA). For Western blotting, Alexa 680-conjugated secondary antibodies (1:5,000) were from Molecular Probes and IRDye 800nm-conjugated secondary antibodies (1:5,000) were from Rockland (Gilbertsville, PA).