Miseq 2500 system

Manufactured by Illumina

Sourced in Germany

The MiSeq 2500 System is a desktop sequencing platform designed for small-scale genomic analysis. It utilizes Illumina's proprietary sequencing-by-synthesis technology to generate high-quality sequencing data. The system is capable of producing up to 15 gigabases of sequence information per run.

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Lab products found in correlation

Miseq platform, by Illumina (1 mentions) Qiaamp fast dna stool mini kit, by Qiagen (1 mentions)

Spelling variants of this product

MiSeq system (1580 citations) MiSeq 2500 (32 citations) HiSeq™2500/MiSeq system (2 citations)

2 protocols using miseq 2500 system

Mouse Gut Microbiome Profiling via 16S rRNA Sequencing

Cited 1 time

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Genomic DNA was isolated from mouse focal samples using the QIAamp Fast DNA Stool Mini Kit purchased from Qiagen (Dusseldorf, Germany), following the instructions provided by the manufacturer. The V4 region of the 16S ribosomal RNA (rRNA) gene was amplified using the forward primer 341F (5'-CCTACGGGAGGCACAG-3') attached to the Roche B adapter for Illumina library construction and the reverse primer 806R (5'-GGACTACNVGGGTWTCTAAT-3') attached to the Roche A adapter and a 10-nt barcode [5'-A-adapter-N (10 (link)) +16S primer-3'].
The DNA libraries were sequenced using the Illumina MiSeq platform, following the instructions provided by manufacturer. Then 16S Ribosomal RNA Gene Amplicons were prepared for the Illumina MiSeq 2500 System. Subsequently, FLASH v1.2.7 software was used to merge the reads and then Trimmomatic v0.33 was used to filter and clean the Tags. After removing chimeras using UCHIME v4.2, the paired-end joined sequences were grouped into operational taxonomic units for subsequent analysis.

Li S., Chen F., Zou Y., Ning L., Zhang G., Zhang S, & Yao Q. (2022). Yinzhihuang oral liquid protects against non-alcoholic steatohepatitis via modulation of the gut-liver axis in mice. Annals of Translational Medicine, 10(11), 631.

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Foot Muscle Transcriptome Analysis

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The whole procedure of RNA extraction, cDNA library construction, and Mi-seq sequencing was already described in great detail in Knobloch et al. (2023) (link). Briefly, a pool of foot muscle preparations of 24 individuals that were collected at freshwater and brackish water locations in Northern Germany and exposed to different salinities under laboratory conditions were used to extract total RNA. Sequencing and assembling were carried out by GATC Biotech AG (Konstanz, Germany) using an Illumina MiSeq2500 system with 300 bp paired-end reads. The sequence reads were adapter trimmed and quality filtered using BBDuk version 34.86 (Bushnell 2015 ). Both raw data (AccNo. SRR15300633) and assembled contigs (AccNo. GKDH00000000.1) were submitted to GenBank.

Fuchs L.I., Knobloch J., Wiesenthal A.A., Fuss J., Franzenburg S., Torres Oliva M., Müller C., Wheat C.W, & Hildebrandt J.P. (2023). A draft genome of the neritid snail Theodoxus fluviatilis. G3: Genes|Genomes|Genetics, 14(3), jkad282.

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