The mEB8 and mouse bone marrow progenitor cells were lysed with RIPA buffer after twice washing with PBS. Then the proteins were quantified by BCA Protein Assay Kit. Primary antibodies used in western blotting were anti-NLRP12 (SAB3500555; Sigma-Aldrich), anti-NIK (AB191592; Abcam), anti-Relb (AB180127; Abcam), anti-ERK1/2 (9102; CST), anti-phospho-ERK1/2 (9101; CST), anti-p38 (9212; CST), anti-phospho-p38 (4511; CST), anti-JNK (9252; CST), anti-phospho-JNK (9255; CST), anti-Gapdh (HC301; Transgen Biotech.) and anti-laminb (66095; Proteintech). The blot was detected with horseradish-peroxidase-labeled anti-rabbit (7074S; CST) or anti-mouse secondary antibody (7076S; CST) and ECL Plus solution (WBKLS0500; Millipore).
Anti gapdh hc301
Manufactured by Transgene
Anti-Gapdh (HC301) is a laboratory reagent used for the detection and quantification of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein in biological samples. GAPDH is a commonly used housekeeping gene and protein marker in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab180127, by Abcam (1 mentions)
Anti lamin b, by Proteintech (1 mentions)
Anti relb, by Abcam (1 mentions)
Ab97040, by Abcam (1 mentions)
Ab11175, by Abcam (1 mentions)
Ab97051, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Ab137034, by Abcam (1 mentions)
P1087, by Beyotime (1 mentions)
Anti β tublin, by Proteintech (1 mentions)
P1008, by Beyotime (1 mentions)
2 protocols using anti gapdh hc301
1
NLRP12 Signaling Pathway Analysis in Bone Marrow Cells
2
Western Blot Analysis of Protein Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed in RIPA lysis buffer (P10013B, Beyotime) with protease inhibitor (P1008, Beyotime) and phosphatase inhibitor (P1087, Beyotime). Total protein concentration was measured by the Bradford assay and separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis, then transferred to polyvinylidene fluoride membrane (Millipore). The membrane was blocked with 5% skimmed milk at room temperature (RT) for 1 h and probed with primary antibodies and subsequently incubated with HRP-conjugated secondary antibodies. The antibodies used in this study are listed as follows. Primary antibodies: anti-Ssrp1 (ab137034, Abcam), anti-GAPDH (HC301, TransGen), anti-β-Tublin (66240-1, Proteintech), anti-phospho-Histone H2A.X (ab11175, Abcam), anti-Spt16 (sc-165987, Santa Cruz). Secondary antibodies: goat anti-mouse IgG-HRP (ab97040, Abcam) and goat anti-rabbit IgG-HRP (ab97051, Abcam).
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