Cells were stained with the appropriate conjugated antibodies for 30 min at 4°C, washed in 0.5% bovine serum albumin/phosphate-buffered saline (PBS) 2 mmol/L EDTA solution, and analyzed using FACSCalibur (BD Biosciences). The following antibodies (anti-human) were utilized: PE CD31: Miltenyi Biotec #130-118-965; FITC CD34: Miltenyi Biotec #130-113-178; APC CD144: Miltenyi Biotec #130-126-010; APC CD73: Miltenyi Biotec #130-124-011; PE CD45 (clone HI30): BioLegend #304008; FITC CD3: Miltenyi Biotec #130-113-690; PE CD90: Miltenyi Biotec #130-117-388; BV421 CD56: BD Horizon #562751; AF647 CD158 (KIR): BD Pharmingen #567324; FITC Perforin: eBioscience #11-9994-42; KO CD45: Beckman Coulter #A96416; PE NKp46: BD Pharmingen #557991; PE-Cy7 CD159α (NKG2A): Beckman Coulter #B10246; PEDazzle CD16: BioLegend #302054; PerCP-Cy5 CD94: BioLegend #302054; FITC IFNγ: Sysmex #AE092283; PE TNFα: BD Pharmingen #554513; CF594 IL-8: BD Horizon #563531.
Pe tnfα
Manufactured by BD
Sourced in United States
PE TNFα is a laboratory reagent used in research applications. It is a fluorescently-labeled antibody that binds to the cytokine tumor necrosis factor alpha (TNFα). This product can be used to detect and quantify TNFα levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd31 pe, by Miltenyi Biotec (1 mentions)
Nkp46 pe, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Gallios instrument, by Beckman Coulter (1 mentions)
Intrapre reagent, by Beckman Coulter (1 mentions)
Il 2 pe, by BD (1 mentions)
Cd3 pe cf594, by BD (1 mentions)
Leukocyte activation cocktail, by BD (1 mentions)
Ifn γ fitc, by BD (1 mentions)
2 protocols using pe tnfα
1
Comprehensive Immunophenotyping of Cell Populations
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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According to the manufacturer’s instructions, peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradients. PBMCs were stimulated with Leukocyte Activation Cocktail (BD Pharmingen, San Diego, CA) at 37 °C for 4 h prior to intracellular staining using the manufacturer’s staining protocol. PBMCs were stained for surface markers, fixed, permeabilized with IntraPreReagent (Beckman Coulter, Fullerton, CA), and then stained with antibodies for intracellular markers. Anti-human mAbs against APC-CD4, FITC-CD56, FITC-IFN-γ, PE-CF594-CD3, PE-IL-2, PE-TNF-α, and V450-CD8, with controls, were purchased from BD Biosciences (San Jose, CA, USA). Data were acquired on a Gallios instrument (Beckman Coulter, Brea, CA) and analyzed with FlowJo software (Ashland, OR).
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