The Drosophila melanogaster Genetic Reference Panel (DGRP) strains were from the Bloomington Drosophila Stock Center (BDSC). The UAS- syp RNAi lines (#33011, #33012) and the genetic control line were from the Vienna Drosophila Resource Center (VDRC). The actin-Gal4 (#4414), the pan-neuronal driver nSyb-Gal4 (#51635), Df(3R)BSC124 (#9289), and Df(3R)BSC141 (#9501) were from the BDSC. Unless stated otherwise, all flies were reared on the standard cornmeal medium from FlyKitchen at the University of Chicago at 25 °C under a 12-hr:12-hr light: dark cycle with the light on at 06:00 and off at 18:00.
Uas syp rnai lines
Manufactured by Vienna Drosophila Resource Center
The UAS-syp RNAi lines are genetic tools used for the knockdown of the synaptotagmin-binding protein (syp) gene in Drosophila melanogaster. These lines allow for targeted and inducible suppression of the syp gene expression, which is involved in the regulation of synaptic vesicle exocytosis and neurotransmitter release. The UAS-syp RNAi lines can be used in combination with the GAL4/UAS system to spatially and temporally control the knockdown of the syp gene in Drosophila.
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2 protocols using uas syp rnai lines
1
Drosophila Genetic Protocols from DGRP
2
Drosophila Genetic Reference Panel
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The Drosophila melanogaster Genetic Reference Panel (DGRP) strains were from the Bloomington Drosophila Stock Center (BDSC). The UAS-Syp RNAi lines (#33011, #33012) and the genetic control line were from the Vienna Drosophila Resource Center (VDRC). The actin-Gal4 (#4414) and the pan-neuronal driver nSyb-Gal4 (#51635) were from the BDSC. Unless stated otherwise, all flies were reared on the standard cornmeal medium from FlyKitchen at the University of Chicago at 25 °C under a 12-hr:12-hr light: dark cycle with the light on at 06:00 and off at 18:00.
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