β actin no 4970

Manufactured by Cell Signaling Technology

Sourced in United States

β-actin (no. 4970) is a highly specific antibody that recognizes the β-actin protein. β-actin is a ubiquitous cytoskeletal protein that is essential for maintaining the structural integrity of cells.

Automatically generated - may contain errors

Lab products found in correlation

Antibodies to glut4 no sc 79838, by Santa Cruz Biotechnology (2 mentions) Pparγ no ab45036, by Abcam (2 mentions) Iblot transfer stack, by Thermo Fisher Scientific (1 mentions) Sds page, by Thermo Fisher Scientific (1 mentions) Np40 cell lysis buffer, by Thermo Fisher Scientific (1 mentions) Anti mouse or anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) N cadherin, by ABclonal (1 mentions) Cxcl1, by ABclonal (1 mentions) Arg 1, by ABclonal (1 mentions) Vimentin, by ABclonal (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

β-actin (6244 citations) β-actin (4970) (56 citations)

5 protocols using β actin no 4970

Western Blotting Antibody Sourcing

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Antibodies to GLUT4 (no. sc-79838) were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). Phospho-AMPK (Thr¹⁷²), PPARα (no. ab8934), and PPARγ (no. ab45036) antibodies were purchased from Abcam Inc. (Cambridge, MA, USA). FAS (no. 3180), phospho-Akt (Ser⁴⁷³) (no. 4060), total AMPK (Thr¹⁷²), phospho-FoxO1 (Ser²⁵⁶) (no. 11115), total-FoxO1 (Ser²⁵⁶) (no. 2880), and β-actin (no. 4970) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Secondary anti-rabbit antibodies were purchased from Jackson ImmunoRes. Lab., Inc. (West Grove, PA, USA).

Lin C.H., Kuo Y.H, & Shih C.C. (2018). Antidiabetic and hypolipidemic activities of eburicoic acid, a triterpenoid compound from Antrodia camphorata, by regulation of Akt phosphorylation, gluconeogenesis, and PPARα in streptozotocin-induced diabetic mice. RSC Advances, 8(37), 20462-20476.

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Western Blot Antibody Validation

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Antibodies to GLUT4 (no. sc-79838) were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). Phospho-AMPK (Thr¹⁷²), PPARα (no. ab8934), and PPARγ (no. ab45036) antibodies were purchased from Abcam Inc. (Cambridge, MA, USA). FAS (no. 3180), phospho-Akt (Ser⁴⁷³) (no. 4060), total AMPK (Thr¹⁷²), and β-actin (no. 4970) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Secondary anti-rabbit antibodies were purchased from Jackson ImmunoRes. Lab., Inc. (West Grove, PA, USA).

Kuo Y.H., Lin C.H, & Shih C.C. (2016). Dehydroeburicoic Acid from Antrodia camphorata Prevents the Diabetic and Dyslipidemic State via Modulation of Glucose Transporter 4, Peroxisome Proliferator-Activated Receptor α Expression and AMP-Activated Protein Kinase Phosphorylation in High-Fat-Fed Mice. International Journal of Molecular Sciences, 17(6), 872.

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Immunoblot Analysis of Signaling Molecules

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The MNC (2 × 10⁷) were resuspended in cell lysis buffer (NP40 Cell Lysis Buffer, Invitrogen) and subjected to SDS-PAGE (Invitrogen). The protein bands were transferred to a nitrocellulose membrane using iBlot® Transfer Stack (Invitrogen) and subjected to immunoblot analysis with the antibodies. Anti-p38 (no. 8690), p-p38 (no. 4511), ERK (no. 4695), p-ERK (no. 4370), p-NF-kB p65 (no. 3033), IkBα (no. 4814), β-actin (no. 4970) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). The alkaline phosphatase (AP) linked anti-rabbit IgG (no. 550321B), anti-mouse IgG (no. 550321A), AP chemiluminescent substrate (no. 1208012) and its enhancer were purchased from Invitrogen.

Wang H., Gao K., Wen K., Allen I.C., Li G., Zhang W., Kocher J., Yang X., Giri-Rachman E., Li G.H., Clark-Deener S, & Yuan L. (2016). Lactobacillus rhamnosus GG modulates innate signaling pathway and cytokine responses to rotavirus vaccine in intestinal mononuclear cells of gnotobiotic pigs transplanted with human gut microbiota. BMC Microbiology, 16, 109.

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Western Blot Protein Expression Analysis

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The protein lysates were prepared using RIPA buffer, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel, transferred to polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA), and probed with primary antibodies at 4 °C overnight. The primary antibodies including vimentin (no. A2666), N-cadherin (no. A0443), Arg-1 (no. A1847), CXCL1 (no. A5802), SOX4 (no. ab80261), p-P65 (no. AP0475), P65 (no. A2547), IKKα (no. A2062), IKKβ (no. A2087) were provided by ABclonal Technology (Cambridge, MA, USA). E-caherin (no. 3195s), p-IKBα (no. 2859), IKBα (no. 4814), p-IKKα/β (no. 2697), β-actin (no. 4970S) and GAPDH (no. 5174s) were purchased form Cell Signaling Technology (Beverly, MA, USA). β-Catenin (sc-7199) and iNOS (bs-0162R, BIOSS) were bought from Santa Cruz (Santa Cruz, CA, USA) and BIOSS (Woburn, MA, USA), respectively. CXCR2 (ab14935) was provided by Abcam (Cambridge, MA, USA). After three washes with Tris-buffered saline with 0.05% Tween-20, the membranes were incubated with secondary anti-rabbit or anti-mouse antibodies (Cell Signaling, MA, USA) for 1 h at room temperature. The signals were visualized using the ECL Advance reagent and quantified using ImageLab software. A triplicate independent experiment was performed.

Wang N., Liu W., Zheng Y., Wang S., Yang B., Li M., Song J., Zhang F., Zhang X., Wang Q, & Wang Z. (2018). CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. Cell Death & Disease, 9(9), 880.

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Osteoclastogenesis Regulation by Ps-GOS

Cited 1 time

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Dulbecco’s Modified Eagle’s Medium (DMEM, No. 11965092) and cell culture reagents were obtained from Gibco (Grand Island, NY, USA). D-pinitol (No. 441252-100MG) and the TRAP staining kit (No 387A-1KT) was purchased from Sigma Aldrich (St. Louis, MO, USA). Recombinant RANKL (No. 462-TEC-010) and M-CSF (No. 416-ML-010) were purchased from R&D Systems (Minneapolis, MN, USA). All specific primers were synthesized by Macrogen (Seoul, Korea). Antibodies for NFATc1 (No. 8032S), cFOS (No. 2250S), NFκB-P65 (No. 8242S), P-NFκB-P65 (No. 3033S) and β-actin (No. 4970S) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies for RANK (No. sc-390655) was provided by Santa Cruz Biotechnology (Dallas, TX, USA). Ps-GOS was received from the Centre for Natural Rubber Latex Biotechnology Research and Innovation Development; its extraction and purification method were detailed in a previous study [26 (link)]. All other reagents and solvents used were supplied by a local company and were of analytical grade.

Rattajak P., Aroonkesorn A., Smythe C., Wititsuwannakul R, & Pitakpornpreecha T. (2024). Pleurotus sajor-caju (Fr.) Singer β-1,3-Glucanoligosaccharide (Ps-GOS) Suppresses RANKL-Induced Osteoclast Differentiation and Function in Pre-Osteoclastic RAW 264.7 Cells by Inhibiting the RANK/NFκB/cFOS/NFATc1 Signalling Pathway. Molecules, 29(9), 2113.

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