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Xenogen ivis lumina 3 in vivo imaging system

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The Xenogen IVIS LUMINA III In Vivo Imaging System is a laboratory equipment designed for the purpose of non-invasive imaging of small animals. It utilizes bioluminescence imaging technology to visualize and quantify biological processes within living subjects.

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Xenogen IVIS Lumina in vivo imaging system IVIS Lumina III In Vivo Imaging System IVIS Lumina in vivo imaging system Xenogen IVIS Lumina imaging system Lumina III in vivo Imaging System Lumina In Vivo Imaging System Xenogen IVIS Lumina system IVIS Lumina III imaging system Xenogen IVIS Lumina IVIS Lumina imaging system

2 protocols using xenogen ivis lumina 3 in vivo imaging system

1

Raji Cell Xenograft Mouse Model

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Six- to 8-week-old male NOD/SCID mice were engrafted with 1 × 106 Raji/ffluc cells via tail vein injection. Three days later, CAR-T or control T cells that had been expanded for 14 days were injected via the tail vein. The number of injected cells was adjusted according to the transduction rate. Animal weights were monitored twice weekly. Tumor progression was evaluated by BLI using a Xenogen IVIS LUMINA III In Vivo Imaging System (PerkinElmer) for up to 28 days.
Ho J.Y., Wang L., Liu Y., Ba M., Yang J., Zhang X., Chen D., Lu P, & Li J. (2021). Promoter usage regulating the surface density of CAR molecules may modulate the kinetics of CAR-T cells in vivo. Molecular Therapy. Methods & Clinical Development, 21, 237-246.
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2

In Vivo Bioluminescence Imaging of Raji-Luc Tumor

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Tumor progression of Raji-Luc was monitored by BLI (bioluminescence imaging) using a Xenogen IVIS LUMINA III in vivo Imaging System (PerkinElmer, Waltham, MA). For bioluminescence in vivo imaging, mice with Raji-Luc engraftment were anesthetized with isoflurane, injected i.p. with D-luciferin (150 mg/kg) and placed into the imaging chamber of an IVIS imaging system. For bioluminescence quantification, a region of interest (ROI) was drawn manually and bioluminescence was recorded as radiance (peak photon/sec/cm2/sr). Levels of bioluminescence for mAb or BiTE treated mice were normalized to levels in PBS treated mice.
Aparicio S., Morrison A., Gould A., Gilthorpe J., Chaudhuri C., Rigby P., Krumlauf R, & Brenner S. (1995). Detecting conserved regulatory elements with the model genome of the Japanese puffer fish, Fugu rubripes.. Proceedings of the National Academy of Sciences of the United States of America, 92(5), 1684-1688.
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