The following vendors were used for the indicated antibodies: Abcam: acetylcholinesterase (ChAT); Cell Signaling Technology: cleaved caspase-3; Covance: microtubule-associated protein (MAP2); Jackson ImmunoResearch Labs: goat anti-rabbit FITC and goat anti-mouse Cy3 antibodies; Millipore: amyloid precursor protein (APP, mab314); Santa Cruz Biotechnologies: MDMx (H-130); Sigma: actin (A2066); ThermoFisher Scientific: horse radish peroxidase (HRP)-conjugated goat anti-mouse and goat anti-rabbit secondary antibodies. The following vendors were used for the indicated chemical reagents: Bachem: Aβ 1–40 peptide; Bio-Rad: Bradford protein assay dye, Polyvinylidene fluoride (PVDF) membrane, prestained broad range molecular weight ladder; Life Technologies: 4–12% Bis-Tris gels, Dulbecco’s Modified Eagle’s Medium (DMEM), Neurobasal media and B27 supplement; Peptide International: Poly-L-Lysine; Scytek Labs: Normal Antibody Diluent; Sigma: bovine serum albumin (BSA), Cytosine β-D-arabinofuranoside, dithiothreitol (DTT), Fast Green FCF, protease inhibitor cocktail, phenylmethanesulfonylfluoride (PMSF), roscovitine, staurosporine; ThermoFisher Scientific: enhanced chemiluminescence (ECL) reagent; Tocris: Q-VD-OPh.
Q vd oph
Manufactured by Bio-Techne
Sourced in United States, United Kingdom
Q-VD-OPh is a broad-spectrum caspase inhibitor that irreversibly binds and inhibits caspase enzymes. It is commonly used in cell culture and biochemical research applications to study apoptosis and cell death pathways.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase, by Thermo Fisher Scientific (1 mentions)
Fast green fcf, by Thermo Fisher Scientific (1 mentions)
Bradford protein assay dye, by Bio-Rad (1 mentions)
Anti brca1 antibody, by Abcam (1 mentions)
Aurora a, by Cell Signaling Technology (1 mentions)
Anti γh2ax ser139, by Merck Group (1 mentions)
Phospho atm ser1981, by Merck Group (1 mentions)
Chloroquine c6628, by Merck Group (1 mentions)
Ku 55 933 s1092, by Selleck Chemicals (1 mentions)
Ku 60019, by Selleck Chemicals (1 mentions)
3 protocols using q vd oph
1
Immunoblotting Protocols for Neurodegenerative Diseases
2
DNA Damage Response Pathway Monitoring
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DDRI-9 (C13H10FN5O4) was provided by the Korea Chemical Bank (Daejeon, Korea) or purchased from either ChemDiv (La Jolla, CA, USA) or Hanchem (Daejeon, Korea). KU55933 (C21H17NO3S2) was purchased from Calbiochem (San Diego, CA, USA). ETO was purchased from Sigma-Aldrich (St. Louis, MO, USA) and Q-VD-OPh was purchased from Tocris (Bristol, UK). The anti-γH2AX (Ser139) and phospho-ATM (Ser1981) antibodies were purchased from Millipore (Temecula, CA, USA). The anti-MDC1, 53BP1, NBS1, H2AX, and phospho-BRCA1 (Ser1423) antibodies were purchased from Bethyl Laboratories (Montgomery, TX, USA). The anti-phospho-DNA-PK antibody (Thr2609) and anti-BRCA1 antibody were purchased from Abcam (Cambridge, UK) and NeoMarkers (Fremont, CA, USA), respectively. The anti-phospho-CHK2 (Thr68), NBS1 (Ser343), CHK2, and Aurora A antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Unless otherwise specified, all other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Small Molecule Inhibitor Testing
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KU-55933 (S1092) and KU-60019 (S1570) were purchased from Selleckchem (Houston, TX, USA). Chloroquine (C6628), bafilomycin (B1793), and 3-methyladenine (3-MA; M9281) were obtained from Sigma Aldrich (St. Louis, MO, USA). Q-VD-OPh was purchased from Tocris (Bristol, UK).
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