Gene Pulser X Electroporator (Bio-Rad, USA) was used to electroporate miR-128-3p into exosomes as previously described [22 (link), 26 (link), 27 (link)]. Briefly, 2 μg exosomes and 400 nmol miR-128-3p mimics were mixed in 400 μl of electroporation buffer at 4 °C. After electroporation at 350 V and 150 μF, the mixture was incubated at 37 °C for 30 mins to fully recover the membrane of exosomes.
Gene pulser x electroporator
Manufactured by Bio-Rad
Sourced in United States
The Gene Pulser X Electroporator is a laboratory instrument designed to facilitate electroporation, a process used for the introduction of exogenous materials, such as nucleic acids, into cells. The core function of the device is to generate controlled electrical pulses that temporarily increase the permeability of cell membranes, enabling the efficient transfer of desired molecules into the target cells.
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Lab products found in correlation
2 protocols using gene pulser x electroporator
1
Exosomal miR-128-3p Electroporation
2
Exosome-Mediated Delivery of circRNA
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The collected serum samples were subjected to centrifugation at 3000g for 15 min to eliminate cells and cellular debris. Exosomes from serum samples were isolated using ExoQuick precipitation kit (SBI, System Biosciences, Mountain View, CA) following the manufacturer’s instructions. Gene Pulser X Electroporator (Bio-Rad, Hercules, CA, USA) was used to electroporate circ_IFT80 or Vector into exosomes as previously described.28 (link) Briefly, exosomes (3 μg) and circ_IFT80 or Vector were mixed in electroporation buffer (400 μL) at 4°C. After electroporation at 350 V and 150 μF in a 4-mm cuvette, the mixture was then incubated for 30 min at 37°C to ensure the exosome membrane was fully recovered. For incubation of exosomes with SW480 and SW620 cells, these cells were co-inoculated into a 12-well plate and exosomes containing circ_IFT80 or Vector were added to per well. SW480 and SW620 cells were collected after incubation for indicated time for further analysis.
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