Some kidney samples, intended for histological examination by light microscopy, were immediately fixed in formalin solution (10%) and processed in a series of graded ethanol solutions. Then, they were embedded in paraffin. Blocks were made and sectioned at a thickness of 5 μm and stained with hematoxylin and eosin, then examined under light microscopy (ZEISS, Axiolab) and were fitted with Canon Power Shot camera (A640) to capture images for histological studies.
Axiolab
Manufactured by Canon
The Axiolab is a microscope system designed for routine laboratory applications. It offers high optical performance and durability for consistent results. The system's core function is to provide clear, detailed images for a variety of laboratory tasks.
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Lab products found in correlation
3 protocols using axiolab
1
Histological Examination of Kidney Samples
2
Copper-Sulfide-Silver Staining of Banana Leaf Melanin
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To melanin staining we follow the procedure proposed by Butler et al. [59] (link). Banana leaves were pretreated for 2 hours in a 10 mM copper sulfate solution in distilled water at room temperature. Subsequently rinsed in 10 ml distilled water for 1 minute, and then were placed in a 1% sodium sulfide keeping at 45–50°C for an hour in dark and after washing in distilled water. Cuts were dried for 10 min and developed in 30 ml solution containing silver lactate 22 mg and 170 mg of hydroquinone in a citrate buffer (0.1 M, pH 3.7) solution for 30 min to 1 hour at 26°C. Treated leaves were mounted directly on slides and examining using bright field microscopy at 40X and 100X. The copper-sulfide-silver procedure gives solid staining over tissue surface, which is purple-black.
All pictures were taken in one session and the image acquisitions were take using light microscope Carl Zeiss Axiolab equipped with a Canon 40D camera adapted to microscope connected to a computer (Imac; Apple).
All pictures were taken in one session and the image acquisitions were take using light microscope Carl Zeiss Axiolab equipped with a Canon 40D camera adapted to microscope connected to a computer (Imac; Apple).
3
Histopathological Analysis of Liver and Kidney
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The liver and kidney tissues were fixed in 10% formalin for 48 h, then dehydrated in graduated ethanol (50-100%) and embedded in paraffin. Sections 5µm thick were made and stained with hematoxylin and eosin dye, then examined for histopathological changes under light microscopy (ZEISS, Axiolab) and fitted with Canon Power Shot camera (A640) to capture images for histological studies. All sections were evaluated to know about for the degree of liver and kidney injury.
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