Purified proteins were obtained by expressing the proteins in an Escherichia coli strain BL21 or commercially. Bacteria were grown in LB medium, induced with 0.25mM isopropyl-β-D-thiogalactoside (IPTG) for 2 hours at 37°C, and cultured at 16°C for 12 hours. The cells were then lysed using appropriate lysis buffer supplemented with Protease Inhibitor Cocktail (Roche). The proteins were purified by binding to appropriate beads for 3 hours at 4°C. The beads were washed with lysis buffer twice and the protein was subsequently eluted. Recombinant human UBA1, UBE2D3/UBCH5C, UBE2N/UBE2V2 complex, UBE2N (UBC13)/UEV1A complex, USP2, RNF8, RNF168, L3MBTL2, and ubiquitin (wild-type and mutants) used for in vitro ubiquitylation assays were purchased from Boston Biochem, Biotechne, Abnova and Origene.
Ube2n ubc13 uev1a complex
Manufactured by R&D Systems
UBE2N (UBC13)/UEV1A complex is a protein complex consisting of the E2 ubiquitin-conjugating enzyme UBE2N and the E2 variant UEV1A. This complex plays a role in the ubiquitination process, which is a post-translational modification that attaches ubiquitin molecules to target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human uba1, by R&D Systems (2 mentions)
Ube2d3 ubch5c, by R&D Systems (2 mentions)
Ube2n ube2v2 complex, by R&D Systems (2 mentions)
Rnf168, by R&D Systems (2 mentions)
L3mbtl2, by R&D Systems (2 mentions)
Ubiquitin, by R&D Systems (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
2 protocols using ube2n ubc13 uev1a complex
1
Purification of Recombinant Ubiquitylation Enzymes
2
Purification of Recombinant Ubiquitylation Enzymes
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Purified proteins were obtained by expressing the proteins in an Escherichia coli strain BL21 or commercially. Bacteria were grown in LB medium, induced with 0.25mM isopropyl-β-D-thiogalactoside (IPTG) for 2 hours at 37°C, and cultured at 16°C for 12 hours. The cells were then lysed using appropriate lysis buffer supplemented with Protease Inhibitor Cocktail (Roche). The proteins were purified by binding to appropriate beads for 3 hours at 4°C. The beads were washed with lysis buffer twice and the protein was subsequently eluted. Recombinant human UBA1, UBE2D3/UBCH5C, UBE2N/UBE2V2 complex, UBE2N (UBC13)/UEV1A complex, USP2, RNF8, RNF168, L3MBTL2, and ubiquitin (wild-type and mutants) used for in vitro ubiquitylation assays were purchased from Boston Biochem, Biotechne, Abnova and Origene.
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