Cells were lysed in radioimmunoprecipitation assay buffer. Cellular proteins were collected and subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and then electrotransferred onto Immobilon-P membranes (Invitrogen; Thermo Fisher Scientific, Inc.). The membranes were then incubated for 2 h at 37°C with rabbit anti-human EGFR (catalog no., sc-367974; 1:1,000 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or rabbit anti-human E-cadherin (catalog no., sc-7870; 1:1,000 dilution; Santa Cruz Biotechnology, Inc.). This was followed by incubation with horseradish peroxidase-conjugated donkey anti-rabbit immunoglobulin G secondary antibody for 1 h at 37°C (catalog no., NA934; 1:1,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ, USA). The rabbit anti-human β-actin antibody (catalog no., sc-7210; 1:1,000 dilution; Santa Cruz Biotechnology, Inc.) was used as an internal marker for control purposes.
Rabbit anti human e cadherin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit anti-human E-cadherin is a primary antibody that recognizes the extracellular domain of the E-cadherin protein expressed in human cells. E-cadherin is a cell-cell adhesion molecule that plays a critical role in the maintenance of epithelial cell-cell junctions and tissue architecture. This antibody can be used for the detection and analysis of E-cadherin expression in various human cell and tissue types.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti human β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Immobilon p membrane, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 donkey anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Dm6000b, by Leica (1 mentions)
α sma, by Bioworld Technology (1 mentions)
2 protocols using rabbit anti human e cadherin
1
Western Blot Analysis of EGFR and E-cadherin
2
Immunofluorescence Staining of E-cadherin and α-SMA
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Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.5% Triton X-100 in PBS, and incubated with rabbit anti human E-cadherin (1:50; Santa Cruz Biotechnology), α-SMA (1:100; Bioworld Technology) at 4 °C overnight. Then, cells were incubated with Alexa Fluor 488 donkey anti-rabbit IgG (H+L) and Alexa Fluor 594 donkey anti-mouse IgG (H+L) (Molecular probes, Eugene, OR, USA) at 37 °C for 1 h. Fluorescent images were captured under a microscope (DM6000B, Leica).
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