For histological analysis, cryosections of teratomas were stained with Gill’s hematoxylin (H-3401; Vector Laboratories, Burlingame, CA, United States) and 0.25% Eosin Y (058-00062; Fujifilm Wako). For toluidine blue staining, sections were stained with 0.05% toluidine blue O (T3260; Sigma-Aldrich) at pH 4.1. Control and induced tenogenic cells at T12 were fixed with 4% PFA in PBS for 30 min. The accumulation of collagen fibers, lipid drops, and cartilaginous matrix was stained with Picrosirius Red (PSR-1; Scy Tek Laboratories, Logan, UT, United States), Oil red O (154-02072; Fujifilm Wako), and Alcian blue 8GX (A3157; Sigma-Aldrich) adjusted at pH 1.0. Alkaline phosphatase (ALP) activity was detected using nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) (11681451001; Roche) staining. Calcification of cells was detected by Alizarin Red S (S5533; Sigma-Aldrich) staining.
Gill s hematoxylin h 3401
Manufactured by Vector Laboratories
Sourced in United States
Gill's hematoxylin (H-3401) is a histological stain used for the visualization of nuclei in tissue sections. It is a commonly used stain in microscopy and pathology laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Alizarin red s, by Merck Group (1 mentions)
Toluidine blue o, by Merck Group (1 mentions)
Toluidine blue, by Merck Group (1 mentions)
Alcian blue 8gx, by Merck Group (1 mentions)
Eosin y, by Fujifilm (1 mentions)
Oil red o, by Fujifilm (1 mentions)
Diaminobenzidine chromogen, by Vector Laboratories (1 mentions)
Avidin biotin complex, by Vector Laboratories (1 mentions)
2 protocols using gill s hematoxylin h 3401
1
Multimodal Histological Characterization of Teratoma and Tenogenic Cells
2
Psoriasin Immunohistochemistry on Bladder Disease Spectrum Tissue Array
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A bladder disease spectrum tissue array (TMA) (BL804) was purchased from US Biomax, Inc. The TMA was de-paraffinised followed by a 20-min incubation with TBS for rehydration. A further 20-min incubation in 0.6% BSA blocking solution was followed by the primary antibody (anti-Psoriasin; dilution 1:100; cat. no. 47C1068; Novus Biologicals, Ltd.) for 1 h at room temperature. After washing, the sections were incubated in the secondary biotinylated antibody (dilution 1:25; biotinylated universal pan-specific antibody, horse anti-mouse/rabbit/goat IgG, cat. no. BA-1300; Vector Laboratories, Inc.) at room temperature for 30 min. An avidin-biotin complex (Vector Laboratories, Inc.) was applied to the TMA and diaminobenzidine chromogen (Vector laboratories, Inc.) was added in the dark for 5 min. Counterstaining of the nuclei was performed using Gill's hematoxylin (H-3401; Vector Laboratories, Inc.). The TMA was dehydrated with increasing grades of methanol, then cleared in xylene and mounted.
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