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Application suite software v 2

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Leica Application Suite software V.2.8.1 is a comprehensive imaging software platform developed by Leica Microsystems. It provides a user-friendly interface for controlling and acquiring images from a variety of Leica microscopes and imaging devices.

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Lab products found in correlation

Ab15086, by Abcam (2 mentions) Dfc420c camera, by Leica (2 mentions)

Close spelling variants of this product

Application Suite V 2.5.0 Application Suite software Leica Applications Suite Leica Application Suite Leica application software Software Suite Leica Application

2 protocols using application suite software v 2

1

Immunohistochemistry for Tumor CA9 Expression

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Immunohistochemistry was performed as previously described on 6 μm slides cut from paraffin-embedded tumours with anti CA9 antibody (1/1500, ab15086, Abcam).15 (link) Antigen retrieval was performed by boiling slides in Tris-EDTA buffer (pH9) for 45 min. Revelation was performed using Histogreen as a chromogen. Images were acquired with a Leica DM400B microscope with Leica Application Suite software V.2.8.1 and a Leica DFC420C camera.
Buffet A., Calsina B., Flores S., Giraud S., Lenglet M., Romanet P., Deflorenne E., Aller J., Bourdeau I., Bressac-de Paillerets B., Calatayud M., Dehais C., De Mones Del Pujol E., Elenkova A., Herman P., Kamenický P., Lejeune S., Sadoul J.L., Barlier A., Richard S., Favier J., Burnichon N., Gardie B., Dahia P.L., Robledo M, & Gimenez-Roqueplo A.P. (2020). Germline mutations in the new E1’ cryptic exon of the VHL gene in patients with tumours of von Hippel-Lindau disease spectrum or with paraganglioma. Journal of Medical Genetics, 57(11), 752-759.
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2

Immunohistochemical Analysis of CA9 in Tumors

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Immunohistochemistry was performed as previously described on 6 µm slides cut from paraffin-embedded tumours with anti CA9 antibody (1/1500, ab15086, Abcam).15 (link) Antigen retrieval was performed by boiling slides in Tris-EDTA buffer (pH9) for 45 min. Revelation was performed using Histogreen as a chromogen. Images were acquired with a Leica DM400B microscope with Leica Application Suite software V.2.8.1 and a Leica DFC420C camera.
Buffet A., Calsina B., Flores S., Giraud S., Lenglet M., Romanet P., Deflorenne E., Aller J., Bourdeau I., Bressac-de Paillerets B., Calatayud M., Dehais C., De Mones Del Pujol E., Elenkova A., Herman P., Kamenický P., Lejeune S., Sadoul J.L., Barlier A., Richard S., Favier J., Burnichon N., Gardie B., Dahia P.L., Robledo M, & Gimenez-Roqueplo A.P. (2020). Germline mutations in the new E1’ cryptic exon of the VHL gene in patients with tumours of von Hippel-Lindau disease spectrum or with paraganglioma. Journal of Medical Genetics, 57(11), 752-759.
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