Hyaluronic acid (HA) with molecular weight 0.5–0.75 MDa was purchased from Contipro. Cystamine dihydrochloride (Cys, Sigma Aldrich, St. Louis, MO, USA), 3,3’-dithiobis(propanoic dihydrazide) (Hyd, FisherScientific, USA) and dithiothreitol (DTT, Fluorochem, Hadfield, United Kingdom) were employed for HA modification with thiol groups. N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, Fluorochem, Had- field, United Kingdom) and 1-Hydroxybenzotriazole hydrate (HOBt, Sigma Aldrich, St. Louis, MO, USA) were used as coupling reagents. Spectra/Por membranes (MWCO: 10 kDa) were employed for purification by dialysis. Phosphate-buffered saline (PBS, Sigma Aldrich P4417, St. Louis, MO, USA) was used as solution medium to form the hydrogels. 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB Ellman’s reagent, Sigma Aldrich, St. Louis, MO, USA), Ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich, St. Louis, MO, USA) and Cysteina-HCl (Thermo fisher Scientific, USA) were used for the determination of thiol groups.
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc
Manufactured by Fluorochem
Sourced in United Kingdom, Italy
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) is a water-soluble carbodiimide that functions as a coupling agent for the formation of amide bonds between carboxylic acids and primary amines.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
1 hydroxybenzotriazole hydrate hobt, by Merck Group (1 mentions)
P4417, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Fluorochem (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb ellman s reagent, by Merck Group (1 mentions)
Triethylamine, by Fluorochem (1 mentions)
Propofol, by Merck Group (1 mentions)
Tetrahydroquinoline, by Merck Group (1 mentions)
Tetrahydroisoquinoline, by Merck Group (1 mentions)
Poly l lys solution 70 150 kda, by Merck Group (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
2 protocols using n 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc
1
Thiol-modified Hyaluronic Acid Hydrogels
2
Synthesis and Characterization of Gabazine-Oregon Green
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Drugs R(+)-Etomidate (etomidate) was purchased from Toronto Research Chemicals Inc. (North York, Ontario, Canada) . The salts used in buffers, HEPES, GABA, propofol, DMEM high glucose, fetal bovine serum, and 0.01% poly-L-Lys solution (70-150 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnenolone sulfate was purchased from Bio-Techne Corp. (Minneapolis, MN, USA) and Sigma-Aldrich. Phenobarbital was obtained from J.T. Baker Chemical Co (Phillipsburg, NJ, USA). Lipofectamine 2000, Opti-MEM, and trypsin-EDTA were obtained from Thermo Fischer Scientific (Waltham, MA, USA). (E)-3-(furan-2-yl)acrylic acid, (E)-3-(2thienyl)acrylic acid, 1-hydroxybenzotriazole hydrate (HOBt), p-toluidine, tetrahydroquinoline, tetrahydroisoquinoline and 1-aminoindoline hydrochloride were obtained from Sigma-Aldrich SRL (Milan, Italy) .
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), triethylamine, sodium hydride and iodomethane were purchased from Fluorochem (Glossop, UK).
Gabazine-oregon green (Gzn-OG) was synthesized as described previously (Sakamoto et al., 2019) .
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), triethylamine, sodium hydride and iodomethane were purchased from Fluorochem (Glossop, UK).
Gabazine-oregon green (Gzn-OG) was synthesized as described previously (Sakamoto et al., 2019) .
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