Agilent lc esi qtof ms ms mass hunter workstation software

The quantification of phenolic compounds present in the berries were executed by Agilent 1200 series HPLC (Agilent Technologies, CA, USA) coupled with a photodiode array detector (PDA) as described by Feng, et al. [29 (link)]. The sample injected was 20 µL and the wavelengths used for detection of the samples were 280 nm, 320 nm, 370 nm. Column and conditions were the same as described in LC-ESI-QTOF-MS/MS analysis. Standard calibration curves were used to detect the compounds found in sample. Data acquisition and analysis were performed using Agilent LC-ESI-QTOF-MS/MS Mass Hunter workstation software (Qualitative Analysis, version B.03.01, Agilent).

The samples’ targeted phenolic compounds were quantified using an Agilent 1200 series HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with a photodiode array (PDA) detector. The same column and conditions were maintained as described above in LC-ESI-QTOF-MS/MS, except for the sample injection volume (20 µL). The compositions of extracts were detected under λ 280 nm, 320 nm, and 370 nm by the PDA detector. The individual polyphenols were quantified based on linear regression of external standards, plotting peak area against concentration. Data acquisition and analysis were performed using the Agilent LC-ESI-QTOF-MS/MS MassHunter workstation software (Qualitative Analysis, version B.03.01, Agilent, Santa Clara, CA, USA).