The following primary antibodies were used for western blotting at 1:1000 dilution: Ago2 (MBL CM004-3), Ago1 (MBL RN028PW), Glucokinase (Abcam ab88056), β-Actin (Sigma A1978), GAPDH (Abcam ab8245), α-Tubulin (Sigma, T6557), Glucose-6-phosphatase (Abcam ab83690), Glycogen synthase (Cell Signaling, #3893), Insulin receptor (Cell Signaling, #3025), Akt2 (Cell Signaling, #2964), phospho-Akt2 (Ser474) (Cell Signaling, #8599), FoxO1 (Cell Signaling, #2880), phospho-FoxO1 (Cell Signaling, #9464), Acetyl-CoA Carboxylase (Cell Signaling, #3676), phospho-Acetyl-CoA Carboxylase (Cell Signaling, #11818), Fatty Acid Synthase (Cell Signaling, #3180), AMPKα (Cell Signaling, #2532), and phospho-AMPKα (Cell Signaling, #2535). Image densitometry of 16-bit TIF images for all western blots was performed using ImageJ.
Phospho akt2 ser474
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-Akt2 (Ser474) is a primary antibody that detects endogenous levels of Akt2 protein when phosphorylated at serine 474.
Automatically generated - may contain errors
Lab products found in correlation
Glycogen synthase, by Cell Signaling Technology (1 mentions)
Insulin receptor, by Cell Signaling Technology (1 mentions)
Acetyl coa carboxylase, by Cell Signaling Technology (1 mentions)
Phospho ampkα, by Cell Signaling Technology (1 mentions)
Phospho acetyl coa carboxylase, by Cell Signaling Technology (1 mentions)
α tubulin, by Merck Group (1 mentions)
Phospho foxo1, by Cell Signaling Technology (1 mentions)
Fatty acid synthase, by Cell Signaling Technology (1 mentions)
Ampkα, by Cell Signaling Technology (1 mentions)
Foxo1, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Gapdh, by Abcam (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Anti total akt antibody, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Santa Cruz Biotechnology (1 mentions)
Phosphoenolpyruvate carboxykinase pepck, by Abcam (1 mentions)
C ebp homologous protein chop, by Cell Signaling Technology (1 mentions)
Phospho eif2a, by Cell Signaling Technology (1 mentions)
Total jnk, by Cell Signaling Technology (1 mentions)
Uncoupling protein 1 ucp1, by Santa Cruz Biotechnology (1 mentions)
2 protocols using phospho akt2 ser474
1
Comprehensive Western Blot Antibody Panel
2
Western Blot Analysis of Cell Signaling
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Immunoblots were done as previously described (Jornayvaz et al. 2012) . Membranes were incubated overnight with primary antibodies for phospho-Akt2 (Ser 474 ) (Cell Signaling Technology, Danvers, MA, USA), phosphoenolpyruvate carboxykinase (PEPCK; Abcam, Cambride, MA, USA), pyruvate carboxylase (PC; Abcam), uncoupling protein 1 (UCP1; Santa Cruz Biotechnology), C/EBP homologous protein (CHOP; Cell Signaling Technology), IgH chain binding protein (BIP; Cell Signaling Technology), phospho-eIF2a (Cell Signaling Technology), or phospho-JNK (Cell Signaling Technology). After further washings, membranes were incubated with HRP-conjugated secondary antibody (Bio-Rad) and visualized by ECL substrate (Pierce, Rockford, IL, USA). Membranes were stripped and reblotted with anti-total Akt antibody (Cell Signaling Technology), total eIF2a (Cell Signaling Technology), total JNK (Cell Signaling Technology), or glyceraldehyde-3-phosphate dehydrogenase (Santa Cruz Biotechnology). Bands were then quantified using ImageJ (National Institutes of Health, Bethesda, MD, USA).
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