Immunofluorescent staining was performed to study the expression of NF-κB subunit p65. Briefly, tissue sections were deparaffinized in xylene and rehydrated in graded ethanols. The tissues were blocked with 5% goat serum for 30 min and incubated with primary antibody mouse monoclonal NF-κB-P65 (F-6) (Santa Cruz Biotechnology, USA) 1:200 for 1.5 h in room temperature. After washing with PBS, slides were incubated with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, USA) and counterstained with DAPI 1:2000 in 1xPBS, and viewed under the IX71 Fluorescence Microscope (Olympus, Japan).
Mouse monoclonal nf κb p65 f 6
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Mouse monoclonal NF-κB-P65 (F-6) is an antibody produced by Santa Cruz Biotechnology. It is designed to target the NF-κB p65 subunit.
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Lab products found in correlation
4 protocols using mouse monoclonal nf κb p65 f 6
1
NF-κB p65 Immunofluorescent Staining
2
NF-κB p65 Immunofluorescent Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immuno uorescent staining was performed to study the expression of NF-κB subunit p65. Brie y, tissue sections were depara nized in xylene and rehydrated in graded ethanols. The tissues were blocked with 5% goat serum for 30 min and incubated with primary antibody mouse monoclonal NF-κB-P65 (F-6) (Santa Cruz Biotechnology, USA) 1:200 for 1.5 hours in room temperature. After washing with PBS, slides were incubated with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (Thermo Fisher Scienti c, USA) and counterstained with DAPI 1:2000 in 1xPBS, and viewed under IX71 Fluorescence Microscope (Olympus, Japan).
3
NF-κB p65 Immunofluorescent Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immuno uorescent staining was performed to study the expression of NF-κB subunit p65. Brie y, tissue sections were depara nized in xylene and rehydrated in graded ethanols. The tissues were blocked with 5% goat serum for 30 min and incubated with primary antibody mouse monoclonal NF-κB-P65 (F-6) (Santa Cruz Biotechnology, USA) 1:200 for 1.5 hours in room temperature. After washing with PBS, slides were incubated with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (Thermo Fisher Scienti c, USA) and counterstained with DAPI 1:2000 in 1xPBS, and viewed under IX71 Fluorescence Microscope (Olympus, Japan).
4
NF-κB p65 Immunofluorescent Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immuno uorescent staining was performed to study the expression of NF-κB subunit p65. Brie y, tissue sections were depara nized in xylene and rehydrated in graded ethanols. The tissues were blocked with 5% goat serum for 30 min and incubated with primary antibody mouse monoclonal NF-κB-P65 (F-6) (Santa Cruz Biotechnology, USA) 1:200 for 1.5 hours in room temperature. After washing with PBS, slides were incubated with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (Thermo Fisher Scienti c, USA) and counterstained with DAPI 1:2000 in 1xPBS, and viewed under IX71 Fluorescence Microscope (Olympus, Japan).
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