Amazon speed spectrometer

Optical rotations were measured with a P2000 automatic digital Polarimeter. UV spectra were recorded on a Hitachi U-3200. IR spectra were recorded on a shimadzu 8900 FT-IR spectrophotometer in KBr disks. NMR spectra were recorded on Bruker Avance-400, Avance-500, Avance-600 MHz using TMS as an internal standard, with chemical shifts recorded as δ values. ESI-MS data were obtained on an ion-trap (Amazon speed) spectrometer (Bruker, Germany) . HRESI-MS were performed on a MicrOTOF-Q mass spectrometer (Bruker) . The EI-MS and HREI-MS were measured on a Jeol JMS-600H mass spectrometer.
Preparative HPLC was carried out on a Japan Analytical Instrument with a LC-908 W detector using a sil-d-60-80A. Column (250 × 20 mm, 4µm) for a normal phase and ODS H-80 column for reverse phase. Analytical thin layer chromatography (TLC) was performed on precoated silica gel plates (Merck 60 PF 254 ; 20 × 20; 0.25 mm) . Circular dichroism was performed on a JASCO J-810 CD spectrophotometer. Column chromatography (CC) was carried out on silica gel (70-230 Mesh; Merck), Sephadex LH-20 (40-70 μm, Amersham Biosciences, Sweden), and vacuum liquid chromatography (VLC) was performed using silica gel 60 (0.04-0.063 mm; 500 g; Merck, Darmstadt, Germany).