Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control. Anti-mouse or anti-rabbit secondary antibodies conjugated to IRDye-800 or IRDye-680 (Li-Cor Biosciences, Lincoln, NE) were detected on an Odyssey TM FC infrared imaging systems (Li-Cor Biosciences).
Odyssey fc infrared imaging
The Odyssey TM FC infrared imaging system is a high-performance instrument designed for sensitive and quantitative detection of fluorescently labeled proteins and nucleic acids. It utilizes dual-channel infrared fluorescence detection to enable simultaneous analysis of multiple targets within a single sample. The system is capable of delivering high-resolution, high-sensitivity images with a wide dynamic range, making it a versatile tool for a variety of applications in the life sciences.
2 protocols using odyssey fc infrared imaging
Yeast Protein Extraction and Detection
Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control. Anti-mouse or anti-rabbit secondary antibodies conjugated to IRDye-800 or IRDye-680 (Li-Cor Biosciences, Lincoln, NE) were detected on an Odyssey TM FC infrared imaging systems (Li-Cor Biosciences).
Yeast Protein Extraction and Detection
Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control. Anti-mouse or anti-rabbit secondary antibodies conjugated to IRDye-800 or IRDye-680 (Li-Cor Biosciences, Lincoln, NE) were detected on an Odyssey TM FC infrared imaging systems (Li-Cor Biosciences).
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