Imagequant tl image analysis software v7

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed on a Multiphor II Electrophoresis System (Pharmacia Biotech, Piscataway, USA), using ExcelGel SDS Homogeneous 15% polyacrylamide gels at 600 V, 20 mA, 20 W and 15 °C (Borreani et al., 2016) . The samples were loaded at a protein concentration of 64 micrograms of protein/well. The protein bands were stained with Coomassie Brilliant Blue tablets (Phastgel Blue R., Pharmacia). De-staining was performed in an aqueous solution of 25% ethanol and 8% acetic acid. The samples were kept in a solution of 10% glycerol and 7.2% acetic acid.
A high molecular weight calibration kit consisting of: phosphorylase b (97 kDa), bovine serum albumin (66 kDa), egg albumin (45 kDa), carbonic anhydrase (30 kDa), trypsin inhibitor (20.1 kDa) and α-lactalbumin (14.4 kDa) (Amersham GE Healthcare, UK) was used as standard.
The gels were scanned with an ImageScanner III LabScan 6.0 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and analyzed with the ImageQuant TL Image analysis Software v7.0 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed by the method of Laemmli (1970) on a Multiphor II Electrophoresis System (Pharmacia Biotech, Piscataway, USA), using 15% polyacrylamide gels (ExcelGel SDS Homogeneous) at 600 V, 18 mA, 18 W and 15 °C. Of each sample, 8 µL were loaded into the gel.
The protein bands were stained with Coomassie Brilliant Blue tablets (Phastgel Blue R., Pharmacia). De-staining was performed in an aqueous solution of 25% ethanol and 8% acetic acid. The samples were preserved in a solution of 10% glycerol and 7.2% acetic acid.
The high molecular weight calibration kit used as the standard (Amersham GE Healthcare, UK) consisted of phosphorylase b (97000 Da), bovine serum albumin (66000 Da), egg albumin (45000 Da), carbonic anhydrase (30000 Da), trypsin inhibitor (20100 Da) and α-lactalbumin (14400 Da).
Gels were scanned with an ImageScanner III LabScan 6.0 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and analysed with the ImageQuant TL Image Analysis Software v7.0 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).