Fly brains were dissected from 3-to-5-day-old adult females and stained exactly as in Wu and Luo (2006) (link). The following primary antibodies were used: mouse anti-HA antibody (HA.11, Covance, BioLegend: 901514) and rabbit anti-GFP antibody (Abcam: ab290) were used at 1:500, and rat anti-Elav antibody (Developmental Studies Hybridoma Bank, Iowa City, IA, deposited by G. M. Ruben: 7E8A10) was used at 1:25. Cross absorbed secondary antibodies used were: goat anti-mouse IgG Alexa Fluor Plus 555 (Invitrogen: A32727), goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen: A11034), and goat anti-rat IgG Alexa Fluor 647 (Invitrogen: A21247). Brains were imaged on an Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope with a 20X objective.
Mouse anti ha antibody ha 11
Manufactured by Fortrea
The Mouse anti-HA antibody (HA.11) is a laboratory reagent used to detect and identify proteins that have been tagged with the HA (Hemagglutinin) epitope. This antibody specifically binds to the HA epitope, allowing researchers to study the expression, localization, and interactions of HA-tagged proteins in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 goat anti rat igg, by Thermo Fisher Scientific (2 mentions)
Rat anti elav antibody, by Developmental Studies Hybridoma Bank (2 mentions)
Goat anti mouse igg alexa fluor plus 555, by Thermo Fisher Scientific (2 mentions)
Lsm 780 multiphoton laser scanning confocal microscope, by Zeiss (2 mentions)
Rabbit anti gfp antibody, by Abcam (2 mentions)
2 protocols using mouse anti ha antibody ha 11
1
Immunostaining of Drosophila Brains
2
Immunostaining of Drosophila Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fly brains were dissected from 3-to-5-day-old adult females and stained exactly as in Wu and Luo (2006) (link). The following primary antibodies were used: mouse anti-HA antibody (HA.11, Covance, BioLegend: 901514) and rabbit anti-GFP antibody (Abcam: ab290) were used at 1:500, and rat anti-Elav antibody (Developmental Studies Hybridoma Bank, Iowa City, IA, deposited by G. M. Ruben: 7E8A10) was used at 1:25. Cross absorbed secondary antibodies used were: goat anti-mouse IgG Alexa Fluor Plus 555 (Invitrogen: A32727), goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen: A11034), and goat anti-rat IgG Alexa Fluor 647 (Invitrogen: A21247). Brains were imaged on an Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope with a 20X objective.
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