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Nexcelom cellometer auto t4 cell counter

Manufactured by Revvity

The Nexcelom Cellometer Auto T4 is an automated cell counter designed for accurately counting cells. It utilizes image-based cytometry technology to provide rapid and precise cell counts. The core function of this product is to enable efficient and reliable cell enumeration for various applications.

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Lab products found in correlation

2 protocols using nexcelom cellometer auto t4 cell counter

1

Growth Factor-Mediated Cell Proliferation

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Cells were maintained in DMEM/F12 containing 10% fetal bovine serum under standard tissue culture conditions. Subconfluent cultures were growth‐arrested for 2 days in DMEM/F12 containing 0.4% bovine serum albumin. Next, cells were placed in DMEM/F12 containing a low concentration of platelet‐derived growth factor–BB (25 ng/mL) and then treated every day for 4 days without or with various treatments. Finally, cells were harvested, and cell number was quantified using a Nexcelom Cellometer Auto T4 cell counter (Nexcelom Bioscience).
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2

Nasal and oropharyngeal sampling for cell analysis

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Nasal lavage fluid was diluted with 3 ml of complete DMEM and oropharyngeal swabs were collected from the distal oropharynx and immediately placed in 2 ml of complete tissue culture medium in 15 ml polypropylene tubes on ice. To dislodge cells from the swabs, the samples were lightly vortexed. Fluid was subsequently transferred to a new tube and the swabs were again rinsed with 2 ml of PBS and pooled with the first wash. Pooled samples were filtered through a 70 μm cell strainer (CellTreat, Pepperell, MA) to remove large debris and mucus. Samples were then centrifuged and the pellets were resuspended in 0.5 ml of PBS and 10 μl aliquots were stained with 0.4% trypan blue and blue-excluding cells were counted using a Nexcelom Cellometer™ Auto T4 cell counter (Nexcelom; Lawrence, MA). Nasal lavage samples were processed similarly. To determine cell types and activation states, equivalent numbers of cells were processed and stained for flow cytometric analyses as described above.
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