Abm mycoplasma pcr detection kit

Manufactured by Applied Biological Materials

Sourced in Canada

The Abm Mycoplasma PCR detection kit is a laboratory tool designed to detect the presence of mycoplasma contamination in cell cultures. The kit utilizes a polymerase chain reaction (PCR) technique to amplify and identify specific mycoplasma DNA sequences. It provides a reliable and sensitive method for monitoring mycoplasma contamination in research and laboratory settings.

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Lab products found in correlation

Crystal violet, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Caisson (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Rwpe 1, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

PCR Mycoplasma Detection Kit (140 citations) Mycoplasma detection kit (10 citations) PCR detection kit (6 citations) Mycoplasma PCR kit (2 citations) Mycoplasma PCR (2 citations)

2 protocols using abm mycoplasma pcr detection kit

Generating IFIT2-depleted Oral Cancer Cells

Cited 1 time

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Sh-control and stable IFIT2-depleted (sh-IFIT2-1 and sh-IFIT2-2) cells were generated in the human oral cancer cell line CAL27 as previously described [31 (link)]. The cells were screened for mycoplasma contamination following the manufacturer’s instructions (abm Mycoplasma PCR detection kit; Cat no. G238 Applied Biological Materials Inc. Richmond BC, Canada) and cultured as previously described [32 (link)]. We also tried to knockdown IFIT2 in other cell lines. However, based on the IFIT2 expression levels, CAL27 cells were apparently the most convenient cells for this study.

Lai K.C., Regmi P., Liu C.J., Lo J.F, & Lee T.C. (2023). IFIT2 Depletion Promotes Cancer Stem Cell-like Phenotypes in Oral Cancer. Biomedicines, 11(3), 896.

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Cell Line Procurement and Characterization

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Cell lines used in this study LNCaP, PC3, RWPE-1 and HEK293T were procured from ATCC (Rockville, MD) between 2004 and 2017. C4-2B cells were generated by Dr. Chung [32 (link)]. These cells were routinely tested for mycoplasma. the latest mycoplasma test, performed on 1/31/2018 using abm-Mycoplasma PCR Detection Kit (Applied Biological Materials Inc. Richmond, BC) as per manufacturer recommendations, was negative. The cell lines were not independently authenticated. L-glutamine, penicillin-streptomycin and sodium pyruvate were purchased from Hyclone/Caisson. Media components and Fetal Bovine Serum (FBS) were procured from Caisson and Atlanta Biologicals respectively and all other chemicals were procured from Corning. Keratinocyte-Serum Free Medium (K-SFM), bovine pituitary extract (BPE) and epidermal growth factor (EGF) were obtained from GIBCO (Germantown, MD). Crystal violet was obtained from Sigma-Aldrich (St. Louis, MO), TET (3B2-0454) and TRAIL (CYT-443) were obtained from 3B Scientific Corporation (Libertyville, IL) and ProSpec-Tany Technogene Ltd. (East Brunswick, NJ) respectively. TRAIL was dissolved in distilled water and kept at −20°C until further use. All other chemicals were purchased from Sigma.

Peters G.A., Hartmann R., Qin J, & Sen G.C. (2001). Modular Structure of PACT: Distinct Domains for Binding and Activating PKR. Molecular and Cellular Biology, 21(6), 1908-1920.

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