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Tragacanth gum

Manufactured by Atlas Biologicals
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Tragacanth gum is a natural gum extracted from the dried sap of certain species of the Astragalus plant. It is a complex polysaccharide composed of galacturonic acid, galactose, xylose, and other sugars. Tragacanth gum is commonly used as a stabilizer, thickener, and emulsifier in various food, pharmaceutical, and cosmetic applications.

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Lab products found in correlation

Tragacanth, by MP Biomedicals (2 mentions) Equafetal, by Atlas Biologicals (2 mentions)

2 protocols using tragacanth gum

1

Plaque Assay for Detecting ZIKV in Mosquito Saliva

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In addition to RT-qPCR, salivary secretions from ZIKV-infected Ae. aegypti were assayed by plaque assay to confirm the presence of infectious virus particles. Briefly, saliva from individual mosquitoes were diluted in cell culture medium and plated on sub-confluent monolayers of Vero cells. Cells were incubated for 1 hr. at 37°C after which a semi-solid medium overlay (Dulbecco’s Modified Eagle Medium with 4% EquaFETAL, 2X Pen-Strep and 4 μg/mL Amphotericin B, mixed with an equal volume of 1.2% Tragacanth gum) (EquaFETAL, Atlas Biologicals, Fort Collins, USA) (Tragacanth, MP Biomedicals, Santa Ana, USA) was added. Cells were incubated for 4–5 days at 37oC in 5% CO2, stained with crystal violet, and plaques enumerated.
Armstrong P.M., Ehrlich H.Y., Magalhaes T., Miller M.R., Conway P.J., Bransfield A., Misencik M.J., Gloria-Soria A., Warren J.L., Andreadis T.G., Shepard J.J., Foy B.D., Pitzer V.E, & Brackney D.E. (2019). Successive Bloodmeals Enhance Virus Dissemination within Mosquitoes and Increase Transmission Potential. Nature microbiology, 5(2), 239-247.
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2

Plaque Assay for Detecting ZIKV in Mosquito Saliva

Check if the same lab product or an alternative is used in the 5 most similar protocols
In addition to RT-qPCR, salivary secretions from ZIKV-infected Ae. aegypti were assayed by plaque assay to confirm the presence of infectious virus particles. Briefly, saliva from individual mosquitoes were diluted in cell culture medium and plated on sub-confluent monolayers of Vero cells. Cells were incubated for 1 hr. at 37°C after which a semi-solid medium overlay (Dulbecco’s Modified Eagle Medium with 4% EquaFETAL, 2X Pen-Strep and 4 μg/mL Amphotericin B, mixed with an equal volume of 1.2% Tragacanth gum) (EquaFETAL, Atlas Biologicals, Fort Collins, USA) (Tragacanth, MP Biomedicals, Santa Ana, USA) was added. Cells were incubated for 4–5 days at 37oC in 5% CO2, stained with crystal violet, and plaques enumerated.
Armstrong P.M., Ehrlich H.Y., Magalhaes T., Miller M.R., Conway P.J., Bransfield A., Misencik M.J., Gloria-Soria A., Warren J.L., Andreadis T.G., Shepard J.J., Foy B.D., Pitzer V.E, & Brackney D.E. (2019). Successive Bloodmeals Enhance Virus Dissemination within Mosquitoes and Increase Transmission Potential. Nature microbiology, 5(2), 239-247.
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