1 2 dioleoyl sn glycero 3 phospho l serine sodium salt

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) is a phospholipid compound. It is a key component of biological membranes and plays a role in various cellular processes.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (7 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Ethylene glycol tetraacetic acid (egta), by Thermo Fisher Scientific (2 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (2 mentions) Oregon green 488 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine dhpe 488, by Avanti Polar Lipids (2 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine sodium salt, by Avanti Polar Lipids (1 mentions) Thapsigargin, by Thermo Fisher Scientific (1 mentions) Fatty acid free bovine albumin bsa, by Thermo Fisher Scientific (1 mentions) Milli q water, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Melittin, by Merck Group (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Texas red labeled 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt tr dhpe, by Thermo Fisher Scientific (1 mentions) Manganese 2 chloride, by Merck Group (1 mentions) Gadobutrol, by Merck Group (1 mentions) Amplex red, by Merck Group (1 mentions)

Close spelling variants of this product

-dioleoyl-sn-glycero-3-phospho-L-serine (5 citations)

14 protocols using 1 2 dioleoyl sn glycero 3 phospho l serine sodium salt

Purification and Production of KRAS4b

Cited 1 time

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Fully processed KRAS4b was produced and purified as described before (85 (link)). All lipids were purchased from Avanti Polar Lipids (Alabaster): 1,2-dioleoyl-sn-glycero-3 phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine sodium salt (DOPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine sodium salt (POPS).

Liang H., Mu H., Jean-Francois F., Lakshman B., Sarkar-Banerjee S., Zhuang Y., Zeng Y., Gao W., Zaske A.M., Nissley D.V., Gorfe A.A., Zhao W, & Zhou Y. (2019). Membrane curvature sensing of the lipid-anchored K-Ras small GTPase. Life Science Alliance, 2(4), e201900343.

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Lipid Membrane Fluorescence Assay

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The lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DOPS) were obtained from Avanti Polar Lipids (Alabaster, AL). Lipid fluorophore 1,2 – Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647 (Atto 647 DOPE) was purchased from Sigma Aldrich (St. Louis, MO). Thapsigargin was used at 2 μM (AC328570010, Thermo Scientific). HEPES, NaCl, EGTA, and fatty acid-free bovine albumin (BSA) were obtained from Fisher Scientific (Rochester, NY). All commercial reagents were used without further purification.

Shukla S., Chen W., Rao S., Yang S., Ou C., Larsen K.P., Hummer G., Hanson P.I, & Hurley J.H. (2023). Mechanism and cellular function of direct membrane binding by the ESCRT and ERES-associated Ca2+-sensor ALG-2. bioRxiv.

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Munc13-1 cDNA Construct Expression

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The lipids used in this study, including 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DOPS), L-α-phosphatidylinositol-4,5-bisphosphate (brain, porcine) (ammonium salt) (PIP₂) were purchased from Avanti Polar lipids.
The cDNA construct with His12–Munc13C (Munc13–1 residues 529 to 1735 with residues 1408 to 1452 replaced by the sequence EF) was expressed in a modified pCMV–AN6 vector, which includes a PreScission cut site following the His12 tag.

Grushin K., Kalyana Sundaram R.V., Sindelar C.V, & Rothman J.E. (2022). Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release. Proceedings of the National Academy of Sciences of the United States of America, 119(7), e2121259119.

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Lipid Preparation for Membrane Studies

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Lyophilized lipids: 1,2-dioleoyl-sn-glycero-3-phospho-l-serine sodium salt (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), Oregon Green 488 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE-488), and 1,2-distearyl-sn-glycero-3-phosphoethanolamine-N-(TopFluor AF488) ammonium
salt (AF488-PE) were purchased from Avanti Polar Lipids (Alabaster
AL).

Makasewicz K., Wennmalm S., Stenqvist B., Fornasier M., Andersson A., Jönsson P., Linse S, & Sparr E. (2021). Cooperativity of α-Synuclein Binding to Lipid Membranes. ACS Chemical Neuroscience, 12(12), 2099-2109.

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Lipid Extraction and Characterization

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The following lyophilized lipids were obtained from Avanti Polar Lipids (Alabaster AL): 1′,3′-bis(1,2-dioleoyl-sn-glycero-3-phospho)-sn-glycerol sodium salt (cardiolipin, CL); 1,2-dioleoyl-sn-glycero-3-phospho-l-serine sodium salt (DOPS); 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC); d-erythrosphingosine; and GM1 and GM3 ganglioside from ovine brain and milk, respectively. Buffer chemicals MES, NaCl, and ThT were of analytical grade, and the water used was ultrapure (<18.2 megohms·cm at 25 °C, MilliQ grade).

Grey M., Dunning C.J., Gaspar R., Grey C., Brundin P., Sparr E, & Linse S. (2014). Acceleration of α-Synuclein Aggregation by Exosomes. The Journal of Biological Chemistry, 290(5), 2969-2982.

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Lipid Vesicle Preparation and Characterization

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1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DOPS) were purchased from Avanti Polar Lipids (USA). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD PE) and Texas red labeled 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (TR-DHPE) were obtained from Invitrogen (China). Sucrose, glucose, HRP, GOD, Amplex Red, melittin, FDA, PI, manganese (II) chloride, and Gadobutrol were purchased from Sigma (China). Cylindrical NdFeb magnets (1T, diameter = 3 cm, thickness = 1 cm) were bought from Gates Qiangci Company (Shanghai, China). The square SS mesh (1 cm × 1 cm) with thickness of 100 μm was custom-made by RGRS Company (Shenzhen, China). Indium tin oxide (ITO) electrode was purchased from Hangzhou Yuhong technology Co. Ltd (China). Millipore Milli-Q water with a resistivity of 18.2 MΩ cm was used in the experiments.

Li Q., Li S., Zhang X., Xu W, & Han X. (2020). Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays. Nature Communications, 11, 232.

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Liophilized Lipids for Membrane Experiments

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Liophilized lipids: 1,2-dioleoyl-sn-glycero-3-phospho-L-serine sodium salt (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), Oregon Green 488 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE-488) and Ganglioside GM1 from ovine brain were purchased from Avanti Polar Lipids (Alabaster AL). Liophilized Atto-647 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-647) was purchased from ATTO-TEC GmbH.

Makasewicz K., Wennmalm S., Linse S, & Sparr E. (2022). α-Synuclein-induced deformation of small unilamellar vesicles. QRB Discovery, 3, e10.

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Lipid Preparation for Membrane Studies

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The lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DOPS) were obtained from Avanti Polar Lipids. DOPE labeled with Atto 647N was purchased from Sigma-Aldrich. HEPES, NaCl, EGTA, and fatty acid-free bovine albumin (BSA) were obtained from Fisher Scientific. All commercial reagents were used without further purification.

Shukla S., Larsen K.P., Ou C., Rose K, & Hurley J.H. (2022). In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair. Proceedings of the National Academy of Sciences of the United States of America, 119(35), e2205590119.

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Lipid Preparation for Membrane Studies

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The following lipids were purchased from Avanti Polar Lipids (USA): Egg L‐alpha‐phosphatidylcholine (EPC); 1,2‐dioleoyl‐sn‐glycero‐3‐phospho‐L‐serine sodium salt (DOPS); 1,2‐dioleoyl‐sn‐glycero‐3‐phospho‐(1′‐myo‐inositol‐3′‐phosphate; PI3P); 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐(Cyanine 5.5; Cy5.5 PE); 1‐Oleoyl‐2‐[12‐[(7‐nitro‐2‐1,3‐benzoxadiazol‐4‐yl)amino]dodecanoyl]‐sn‐Glycero‐3‐Phosphocholine (NBD‐PC). All lipids were dissolved in chloroform. Phosphatidylinositol phosphates were dissolved in chloroform/methanol/water (20:10:1). Texas red DHPE (Thermo Fisher cat. T1395MP) was purchased as a mixed isomer. The para isomer was separated by thin‐layer chromatography as previously described (Dar et al, 2015 (link)).

Gopaldass N., De Leo M.G., Courtellemont T., Mercier V., Bissig C., Roux A, & Mayer A. (2023). Retromer oligomerization drives SNX‐BAR coat assembly and membrane constriction. The EMBO Journal, 42(2), e112287.

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Reconstitution of Lipid Bilayer Systems

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1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (sodium salt) (DOPS), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine
B sulfonyl) (ammonium salt) (LRB-DOPE) were from Avanti Polar Lipids
(Alabaster, AL); Alexa Fluor 488 C2-maleimide and N-[5-(dimethylamino)naphthalene-1-sulfonyl]-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (dansyl-PE) were from Life
Technologies. 2-Mercaptoethanol (BME) was from Fisher. All reagents
were ACS grade or higher.

Vasquez J.K., Chantranuvatana K., Giardina D.T., Coffman M.D, & Knight J.D. (2014). Lateral Diffusion of Proteins on Supported Lipid Bilayers: Additive Friction of Synaptotagmin 7 C2A–C2B Tandem Domains. Biochemistry, 53(50), 7904-7913.

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