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Hela human cervical cancer cell

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HeLa human cervical cancer cells are a widely used immortalized cell line derived from the cervical cancer cells of Henrietta Lacks. The cells are used extensively in biomedical research and have been crucial in the development of numerous medical advancements.

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Lab products found in correlation

A549 human lung adenocarcinoma cells, by American Type Culture Collection (2 mentions) Hct116 human colon cancer cells, by American Type Culture Collection (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Hek293 human embryonic kidney cells, by American Type Culture Collection (1 mentions) Pc 12 rat pheochromocytoma cells, by American Type Culture Collection (1 mentions) H1299, by American Type Culture Collection (1 mentions) Erythro 9 2 hydroxy 3 nonyl adenine ehna hydrochloride, by Bio-Techne (1 mentions) Mcf 7 human breast cancer cells, by American Type Culture Collection (1 mentions) Hek293t embryonic kidney cells, by American Type Culture Collection (1 mentions) 4t1 mouse mammary carcinoma cells, by American Type Culture Collection (1 mentions) Mda mb 231 human breast cancer cells, by American Type Culture Collection (1 mentions) Nih 3t3 fibroblasts, by American Type Culture Collection (1 mentions) Egm 2 bulletkit, by Lonza (1 mentions) Rpmi8226, by American Type Culture Collection (1 mentions) Mm 1s, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Pc3 and du145 human prostate cancer cells, by American Type Culture Collection (1 mentions)

15 protocols using hela human cervical cancer cell

1

Culturing Diverse Human and Rodent Cell Lines

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A549 human lung adenocarcinoma cells, H1299 human non-small cell lung carcinoma cells, HeLa human cervical cancer cells, NT2 human teratocarcinoma cells, and HEK293 human embryonic kidney cells were obtained from ATCC (Rockville, MD, USA) and grown in Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% FBS and penicillin/streptomycin in a humidified atmosphere with 5% CO2. HL-60 human promyelocytic leukemia cells and PC-12 rat pheochromocytoma cells were obtained from ATCC (Rockville, MD, USA) and grown in RPMI-1640 medium supplemented with 10% FBS and penicillin/streptomycin or RPMI-1640 medium supplemented with 10% horse serum, 5% FBS, and penicillin/streptomycin. 3T3-L1 mouse fibroblasts were obtained from the Japanese Collection of Research Bioresource Bank (Tokyo, Japan) and grown in DMEM supplemented with 10% bovine calf serum and penicillin/streptomycin.
Kamada R., Tano F., Kudoh F., Kimura N., Chuman Y., Osawa A., Namba K., Tanino K, & Sakaguchi K. (2016). Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells. PLoS ONE, 11(8), e0160625.
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2

Stable Isotope-Labeled Nucleotide Synthesis

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All chemicals and enzymes, unless otherwise specified, were purchased from Sigma-Aldrich (St. Louis, MO) and New England Biolabs (Ipswich, WA). Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) hydrochloride was purchased from Tocris Bioscience (Ellisville, MO). 15N3-cytidine-5′-triphosphate was obtained from Sigma-Aldrich (St. Louis, MO) and all other stable isotope-labeled nucleoside starting materials were from Cambridge Isotope Laboratories (Tewksbury, MA). Mouse tissues were obtained from 19-21 week old animals. The HEK293T embryonic kidney cells, MCF7 human breast cancer cells,HCT116 human colon cancer cells, HeLa human cervical cancer cells, WM-266-4 human melanoma cells, and cell culture reagents were purchased from ATCC (Manassas, VA).
Fu L., Amato N.J., Wang P., McGowan S.J., Niedernhofer L.J, & Wang Y. (2015). Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography-Tandem Mass Spectrometry Coupled with the Stable Isotope-dilution Method. Analytical chemistry, 87(15), 7653-7659.
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3

Cell Culture Maintenance Protocol

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HeLa human cervical cancer cells (ATCC CCL-2) and NIH-3T3 fibroblasts (ATCC CRL-1658) were maintained in Dulbecco’s Modified Eagle’s Medium (Hyclone, South Logan, UT, USA), and 4T1 mouse mammary carcinoma cells (ATCC CRL-2539), MDA-MB-231 human breast cancer cells (ATCC HTB-26), Raw264.7 and J774A.1 mouse macrophage cells (ATCC TIB-71 and ATCC TIB-67), and NCI-N87 human gastric cancer cells (ATCC CRL-5822) were maintained in RPMI medium (Hyclone). The media were supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin-streptomycin (Hyclone). Human umbilical vein endothelial cells (ATCC CRL-1730) were maintained in endothelial cell growth medium, EGM-2 Bulletkit (Lonza). All cells were cultured in tissue culture flasks in a humidified incubator at 37 °C in an atmosphere of 95% air and 5% carbon dioxide.
Kim H., Lee J., Oh C, & Park J.H. (2017). Cooperative tumour cell membrane targeted phototherapy. Nature Communications, 8, 15880.
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4

Cell Line Characterization and Maintenance

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All human MM cell lines (RPMI8226, MM.1S, KMS20, and AMO1) were purchased from ATCC. Bortezomib resistant AMO1 cell line (AMO1-VR) was a kind gift of Dr. Christopher Driessen, Kantonsspital St. Gallen, Switzerland. All MM cell lines were maintained in Roswell Park Memorial Institute (RPMI)-1640 medium containing 2.5 mg/mL plasmocin, 1 × Gibco Antibiotic–Antimycotic (100 U/mL of penicillin, 100 μg/mL of streptomycin, and 25 ng/mL of Gibco Amphotericin B), supplemented with 10% (v/v) fetal bovine serum (FBS) and 2 μg L-glutamine. HeLa human cervical cancer cells were purchased from ATCC, cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, 21,063–029) supplemented with 10% fetal bovine serum and 1% 100× penicillin–streptomycin solution. All cell lines were authenticated via short tandem repeat (STR) profiling performed by the Dana Farber Cancer Institute Molecular Diagnostic Laboratory. Mycoplasma testing was routinely performed prior to experiments.
Liu X., Moscvin M., Oh S., Chen T., Choi W., Evans B., Rowell S.M., Nadeem O., Mo C.C., Sperling A.S., Anderson K.C., Yaqoob Z., Bianchi G, & Sung Y. (2023). Characterizing dry mass and volume changes in human multiple myeloma cells upon treatment with proteotoxic and genotoxic drugs. Clinical and experimental medicine, 23(7), 3821-3832.
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5

Culturing Human and Mouse Cancer Cells

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MDA-MB-231, T47D, MCF7, and MDA-MB-468 human breast cancer cells, PC3 and DU145 human prostate cancer cells, and HeLa human cervical cancer cells were obtained from the American Type Culture Collection (Rockville, MD, USA). All human cell lines were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum and to 100 units/mL of streptomycin and penicillin (Hyclone, Logan, UT, USA). 4T1.2 mouse mammary carcinoma cells were maintained in MEM-alpha media supplemented with 10% fetal bovine serum and to 100 units/mL of streptomycin and penicillin. All cells and experimental incubations were maintained at 37° C, 5% carbon dioxide, and 100% humidity in a water-jacketed cell culture incubator.
Tawara K., Scott H., Emathinger J., Wolf C., LaJoie D., Hedeen D., Bond L., Montgomery P, & Jorcyk C. (2019). HIGH expression of OSM and IL-6 are associated with decreased breast cancer survival: synergistic induction of IL-6 secretion by OSM and IL-1β. Oncotarget, 10(21), 2068-2085.
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6

Cell Culture Protocol for Cancer Research

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HEK293T human embryonic kidney cells, HeLa human cervical cancer cells, MCF-7 human breast adenocarcinoma cells, HCT116 human colon carcinoma cells, HepG2 human liver cancer cells, and U2OS human osteosarcoma cells were purchased from American Type Culture Collection (ATCC, Manassas, USA). Cells were grown at 37°C with 5% CO
2 in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, USA) containing 10% fetal bovine serum (Sijiqing, Beijing, China), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, St Louis, USA). We used PCR to detect mycoplasma contamination and confirmed that there was no contamination for any cell lines used in this study.
Yang H., Jiang T., Fan L, & Qiu X. (2023). lncRNA LINC00960 promotes apoptosis by sponging ubiquitin ligase Nrdp1-targeting miR-183-5p: LINC00960 promotes apoptosis by Nrdp1/miR-183-5p. Acta Biochimica et Biophysica Sinica, 55(1), 91-102.
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7

HeLa Cell Culture Protocol

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HeLa human cervical cancer cells were from American Type Culture Collection (ATCC, Manassas, VA). Control, TMEM97 KO, PGRMC1 KO and TMEM97/PGRMC1 double KO HeLa cell lines were grown in MEM containing 10% fetal bovine serum, 2 mM l-glutamine, 1% non-essential amino acids, 100 units/ml penicillin, and 100 μg/ml streptomycin. All the cell lines were maintained at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Zeng C., Weng C.C., Schneider ME J.r., Puentes L., Riad A., Xu K., Makvandi M., Jin L., Hawkins W.G, & Mach R.H. (2019). TMEM97 and PGRMC1 do not mediate sigma-2 ligand-induced cell death. Cell Death Discovery, 5, 58.
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8

Culturing HeLa and DF-1 cells for NDV infection

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HeLa human cervical cancer cells and the DF-1 chicken fibroblast cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Hyclone, UT, USA), penicillin (100 units/ml) and streptomycin (100 μg/ml) (Invitrogen, USA) at 37°C in a 5% CO2 incubator.
The NDV strain Herts/33 was purchased from China Institute of Veterinary Drug Control (Beijing, China). The viruses were propagated in embryonated chicken eggs and titrated on DF-1 cells. Virus was used for infection at multiplicity of infection (MOI) of 1 throughout this study.
Liao Y., Wang H.X., Mao X., Fang H., Wang H., Li Y., Sun Y., Meng C., Tan L., Song C., Qiu X, & Ding C. (2017). RIP1 is a central signaling protein in regulation of TNF-α/TRAIL mediated apoptosis and necroptosis during Newcastle disease virus infection. Oncotarget, 8(26), 43201-43217.
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9

Cell Line Establishment and Maintenance

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BABL-3T3 mouse embryonic fibroblasts cells, A549 human lung adenocarcinoma cells, HeLa human cervical cancer cells, and SKOV3 human ovarian cancer cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The BABL-3T3 cell line was cultured in DMEM (high glucose) and other cells were grown in DMEM (low glucose) containing 10% fetal bovine serum. HUVECs were isolated from term umbilical cord veins using collagenase and cultured in DMEM supplemented with 20% fetal bovine serum. All cell lines were grown at 37 °C in a humidified 5% CO2 atmosphere. HUVEC cells were used within 6 passages.
Wang J., Chen D., Li B., He J., Duan D., Shao D, & Nie M. (2016). Fe-MIL-101 exhibits selective cytotoxicity and inhibition of angiogenesis in ovarian cancer cells via downregulation of MMP. Scientific Reports, 6, 26126.
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10

Maintenance of HEK293A, HeLa, and HepG2-ISRE-Luc2 Cell Lines

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Human embryonic kidney 293A (HEK293A) cells (Qbiogene, Carlsbad, CA, USA) and HeLa human cervical cancer cells (American Type Culture Collection, Manassas, VA, USA) were maintained in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum (Invitrogen) and 1% penicillin/streptomycin at 37°C in a 5% CO2 atmosphere. The HepG2-ISRE-Luc2 cell line was established and maintained as previously reported [17 (link)].
He Y., Huang J., Wang P., Shen X., Li S., Yang L., Liu W., Suksamrarn A., Zhang G, & Wang F. (2015). Emodin potentiates the antiproliferative effect of interferon α/β by activation of JAK/STAT pathway signaling through inhibition of the 26S proteasome. Oncotarget, 7(4), 4664-4679.
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