Daniovision device

Locomotor behavior (distance traveled and velocity) was measured in homozygous epm2a^−/− F2 larvae and WT at five dpf using the Daniovision device (Noldus Information Technology, Wageningen, The Netherlands). Briefly, single larvae were transferred into 96-well plates containing 300 μL of E3 medium per well. Then, one plate at a time was placed in the DanioVision system, and larval locomotor activity was recorded for 30 min and analyzed using EthoVision XT software (Noldus Information Technology, Wageningen, The Netherlands). Coiling behavior was measured to assess burst activity in homozygous epm2a^−/− and WT embryos at 30 hpf; to this end, the number of tail flicks was measured in 30-s time frames using Danioscope software (Noldus Information Technology, Wageningen, The Netherlands).

Coiling behavior was measured in 30 hpf embryos using Danioscope software (Noldus^©, the Netherlands). We also analyzed locomotion in 120 hpf larvae in each experimental group. The larvae were transferred into 96-well plates containing 300 μL of egg water per well. Each plate was placed in the DanioVision^® device (Noldus^© Information Technologies, Wageningen, The Netherlands) and the larval activity was recorded for 30 min and analyzed using EthoVision XT^® software (Noldus^© Information Technologies, Wageningen, The Netherlands) [79 (link)]. Statistical analysis was performed considering five independent biological replicate experiments and the data were plotted as the mean ± standard error of the mean.