C666 1

Manufactured by American Type Culture Collection

Sourced in United States

The C666-1 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating and concentrating a variety of biological samples, including cells, organelles, and macromolecules. The centrifuge features adjustable speed and time settings, allowing users to optimize the separation process for their specific needs.

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Lab products found in correlation

23 protocols using c666 1

Regulation of IGF-1R by Let-7g-5p in NPC cells

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The nasopharyngeal epithelial cell line NP69, NPC cell lines SUNE1, C666-1, 6-10B, and HNE-3 were purchased from ATCC (USA). Cells were cultured in DMEM medium containing 10% FBS and 1% penicillin-streptomycin at 37°C in an incubator with 5% CO₂. Culture related reagents were purchased from GIBCO Invitrogen (USA).
The targets of Let-7g-5p predicted by computer-aided algorithms were obtained from TargetScan, microRNA and miRBase. The Let-7g-5p mimics were transfected into the cells by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol. Empty vector was used as negative control.
The IGF-lR cDNA was subcloned into pcDNA3.1 (Sangon Biotech, China) to construct a pcDNA-IGF-1R expression vector. IGF-1R transfection was performed using Lipofectamine 2000 following the manufacturer’s protocol.

Zhao Z., Wen J., Peng L, & Liu H. (2019). Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 5747-5756.

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Maintenance of EBV-positive and EBV-negative Cell Lines

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EBV⁺ cell lines, including B95-8 (EBV transformed lymphocyte, ATCC: CRL-1612), C666-1 (Human NPC cell line, CVCL_7949) and CNE-2Z (Human NPC cell line, CVCL_6890) were respectively obtained from American Type Culture Collection (ATCC) and Guangzhou Taisheng Bio-Tech Co. Ltd (Guangzhou, China). EBV-negative melanoma cell line of A375 (ATCC: CRL-1619) was obtained from ATCC. The cells were grown in either RPMI-1640 medium (B95-8, C666-1 and CNE-2Z) or high glucose Dulbecco’s modified Eagle’s medium (DMEM) (A375) supplemented with 10% fetal bovine serum (FBS), 100 units/mL of penicillin, and 0.1 mg/mL streptomycin. All cell lines were maintained by serial passage in 5% CO₂ incubator at 37°C.

Zhu S., Chen J., Xiong Y., Kamara S., Gu M., Tang W., Chen S., Dong H., Xue X., Zheng Z.M, & Zhang L. (2020). Novel EBV LMP-2-affibody and affitoxin in molecular imaging and targeted therapy of nasopharyngeal carcinoma. PLoS Pathogens, 16(1), e1008223.

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Culturing Nasopharyngeal Cancer Cells

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The nasopharyngeal cancer cell line, C666-1, was purchased from ATCC and grown in RPMI-1640 supplemented with 10% FBS, 100 μg/mL penicillin, and 100 μg/mL streptomycin. The cells were maintained in a humidified incubator with 5% CO₂ at 37 ℃.

Jiang C., Feng D., Zhang Y., Yang K., Hu X, & Xie Q. (2023). SCAT8/miR-125b-5p axis triggers malignant progression of nasopharyngeal carcinoma through SCARB1. BMC Molecular and Cell Biology, 24, 15.

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Culturing Nasopharyngeal Carcinoma Cell Lines

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Human Nasopharyngeal carcinoma (NPC) cell lines (CNE2, HNE2, 6-10B, H0NE1, HK1, and C666-1) were purchased from the ATCC (Manassas, VA, United States) and maintained in our laboratory. Cell lines were maintained at 37 °C in a 5% CO₂ atmosphere in Roswell Park Memorial Institute (RPMI) 1640 (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (Gibco Life Technologies, Grand Island, NY, USA).

He Z., Yue C., Chen X., Li X., Zhang L., Tan S., Yi X., Luo G, & Zhou Y. (2023). Integrative Analysis Identified CD38 As a Key Node That Correlates Highly with Immunophenotype, Chemoradiotherapy Resistance, And Prognosis of Head and Neck Cancer. Journal of Cancer, 14(1), 72-87.

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Culturing Human HNSCC Cell Lines

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Human HNSCC cell lines FADU, SCC15, NPC/HK1, C666-1, SNU-46, SNU-899 were purchased from ATCC. FADU, SNU-46 and SNU-899 were cultured in DMEM (Gibco) containing 10% fetal bovine serum and 1.0 mmol/L penicillin-streptomycin combination (Hyclone). SCC15, NPC/HK1, and C666–1 were cultured in 1640 medium added in 10% fetal bovine serum. All cell lines were incubated in a humidified atmosphere at 37 °C containing 5% CO₂.

Zheng M., Wang Z., Li M., Yang N., Lu H., Zhang Z., Dong Y., Chen Y., Zhu Z., Tong A, & Yang H. (2024). A novel SLC3A2-targeting antibody-drug conjugate exerts potent antitumor efficacy in head and neck squamous cell cancer. Translational Oncology, 45, 101981.

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HPV-Positive Cell Lines Protocol

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SiHa (ATCC: HTB-35, HPV16 positive), CaSki (ATCC: CRL-1550, HPV16 positive), and C666-1 (ATCC: CVCL_7949, used as HPV16 negative control cell line) were obtained from the American Type Culture Collection and cultured as previously described [6 (link),18 (link)].

Zhang Q., Zhu H., Cui Z., Li Y., Zhuo J., Ye J., Zhang Z., Lian Z., Du Q., Zhao K.N., Zhang L, & Jiang P. (2022). The HPV16E7 Affibody as a Novel Potential Therapeutic Agent for Treating Cervical Cancer Is Likely Internalized through Dynamin and Caveolin-1 Dependent Endocytosis. Biomolecules, 12(8), 1114.

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Culturing C666-1 Human NPC Cells

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The human NPC cell line of C666-1 was obtained from the American Type Culture Collection (ATCC, Manassas, U.S.A.). Cell lines were cultured in RPMI-1640 medium from Gibco (Carlsbad, CA, U.S.A.) supplemented with 10% FBS (Gibco) at 37°C in 5% CO₂.

Zhang Y., Cao Y., Zhang L., Feng C., Zhou G, & Wen G. (2018). Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR. Bioscience Reports, 38(3), BSR20180456.

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Activation of Wnt Signaling in NPC Cells

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Human nasopharyngeal epithelial cell line (NP69, as a control) and NPC cell lines (C666-1, 5-8F and SUNE-1) were purchased from the American Type Culture Collection (Manassas, MA, USA), and cultured in 5% CO₂ at 37°C. Dulbecco's modified Eagle's medium (DMEM) was used as basic culture medium. 10% fetal bovine serum (FBS), 100 U/ml streptomycin and 100 µg/ml penicillin were then added in to the medium. DMEM, FBS, streptomycin and penicillin were purchased from Biological Industries (Kibbutz Beit Haemek, Israel). Lithium chloride (LiCl; 20 mM; incubation time 24 h) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) and was used to activate the Wnt signaling pathway as previously described (31 (link)).

Chen C, & Shan H. (2019). Keratin 6A gene silencing suppresses cell invasion and metastasis of nasopharyngeal carcinoma via the β-catenin cascade. Molecular Medicine Reports, 19(5), 3477-3484.

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Nasopharyngeal Carcinoma Tissue Analysis

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Human nasopharyngeal carcinoma tissues (n = 15) and normal nasopharyngeal epithelial tissues (n = 39) were obtained from patients diagnosed at hospital. The protocol for the use of human tissues was approved by the Institutional Research Ethics Committee in Hospital, and patients’ consent was obtained prior to the surgical resection. All specimens were snap‐frozen in liquid nitrogen and stored at −80 °C for subsequent use. Human nasopharyngeal epithelial cell line NP69 and nasopharyngeal carcinoma cell lines CNE‐1, CNE‐2, HONE‐1, HNE‐1, C666‐1 and SUNE‐1 were purchased from American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI‐1640 supplemented with 5% FBS at 37 °C in an atmosphere of 5% CO₂. The experiments were undertaken with the informed written consent of each subject. The study methodologies conformed to the standards set by the Declaration of Helsinki.

Yin W., Shi L, & Mao Y. (2018). MiR‐194 regulates nasopharyngeal carcinoma progression by modulating MAP3K3 expression. FEBS Open Bio, 9(1), 43-52.

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Comparative Analysis of NPC Cell Lines

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Human NPC cell lines (5-8F, 6-10B, C666-1, CNE1, CNE2, HNE-1, HONE-1, and SUNE-1) were purchased from American Type Culture Collection (ATCC) and maintained in RPMI-1640 (Invitrogen, Grand Island, NY, USA). Human nasopharyngeal epithelial cell line NP69 was obtained from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China) and cultured in keratinocyte-SFM (Invitrogen). HEK-293T cells were purchased from ATCC and grown in DMEM (Invitrogen). A total of 67 NPC tissues were obtained from patients who received primary surgery at Zhuji People’s Hospital. Twelve normal nasopharyngeal epithelial tissues were collected from patients who had nasal operations. Our study protocol was approved by the research ethics committee of Zhuji People’s Hospital (ZJSRMYY-2017H-052). Written informed consent was signed by all participants.

Li H., Huang J., Yu S, & Lou Z. (2020). Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma. OncoTargets and therapy, 13, 2037-2045.

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