HIF-1α inhibitor (EF-24), 7-aminoactinomycin D (7-AAD), rhodamine 123, and nicardipine were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). These compounds were dissolved in dimethyl sulfoxide (DMSO) or ethanol, and the final concentration of DMSO or ethanol in the controls and in each sample did not exceed 0.1%. We found that 0.1% DMSO or ethanol did not affect the cell growth rate compared with 0% DMSO or ethanol (no treatment) in breast cancer cells (data not shown). The STAT3 inhibitor (S3I-201) was obtained from Calbiochem (San Diego, CA, U.S.A.). JAK inhibitor I was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, U.S.A.). Annexin V, Alexa Fluor® 488 Conjugate was obtained from Thermo Fisher Scientific Korea (Seoul, Korea). An EZ-western chemiluminescent detection kit was purchased from Daeillab Service Co. (Seoul, Korea).
Ez western chemiluminescent detection kit
Manufactured by Daeil Lab Service
The EZ-western chemiluminescent detection kit is a lab equipment product designed for Western blot analysis. It enables the detection of target proteins through the use of chemiluminescent signals.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 alexa fluor 488 conjugate, by Thermo Fisher Scientific (2 mentions)
Nicardipine, by Merck Group (2 mentions)
Jak inhibitor 1, by Santa Cruz Biotechnology (2 mentions)
Ef 24, by Merck Group (2 mentions)
Apigenin, by Merck Group (2 mentions)
Z ietd fmk, by R&D Systems (2 mentions)
Z lehd fmk, by R&D Systems (2 mentions)
7 aminoactinomycin d 7 aad, by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
4 protocols using ez western chemiluminescent detection kit
1
Investigating Cancer Cell Signaling Pathways
2
Apigenin Inhibits HIF-1α and STAT3 Pathways
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Apigenin (4′,5,7-trihydroxyflavone), HIF-1α (hypoxia-inducible factor 1-α) inhibitor (EF-24), 7-aminoactinomycin D (7-AAD), rhodamine 123 and nicardipine were purchased from Sigma Chemical Co. (St. Louis, MO, USA). These compounds were dissolved in dimethyl sulfoxide (DMSO) or ethanol, and the final concentration of DMSO or ethanol in the controls and in each sample did not exceed 0.1%. The STAT3 inhibitor (S3I-201) was purchased from Calbiochem (San Diego, CA, USA). JAK (Janus kinase) inhibitor I was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Annexin V, Alexa Fluor® 488 Conjugate was purchased from Thermo Fisher Scientific Korea (Seoul, Korea). An EZ-western chemiluminescent detection kit was obtained from Daeillab Service Co. (Seoul, Korea).
3
Apigenin, FCCP, and EF-24 in Breast Cancer
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Apigenin (4',5,7-trihydroxyflavone), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and HIF-1α inhibitor (EF-24) were purchased from Sigma Chemical Co. These compounds were dissolved in dimethyl sulfoxide (DMSO) and the final concentration of DMSO in the controls and each sample did not exceed 0.1%. We found that 0.1% DMSO did not affect the cell growth rate compared with 0% DMSO (no treatment) in breast cancer cells (data not shown). JC-1 was obtained from Molecular Probes (Invitrogen). The caspase-8 inhibitor Z-IETD-fmk and the caspase-9 inhibitor Z-LEHD-fmk were obtained from R&D Systems, Inc. The STAT3 inhibitor S3I-201 was obtained from Calbiochem. The JAK inhibitor I was purchased from Santa Cruz Biotechnology, Inc. An EZ-western chemiluminescent detection kit was purchased from Daeillab Service Co.
4
Quercetin and FCCP Modulate Mitochondrial Dynamics
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Quercetin (3,3′,4′,5,6-pentahydroxyflavone) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). These compounds were dissolved in dimethyl sulfoxide (DMSO), and the final concentration of DMSO in the controls and each sample did not exceed 0.1%. We found that 0.1% DMSO did not affect the cell growth rate compared to 0% DMSO (no treatment) in the breast cancer cells (data not shown). JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) was obtained from Molecular Probes (Invitrogen, Carlsbad, CA, USA). The caspase-8 inhibitor Z-IETD-fmk and the caspase-9 inhibitor Z-LEHD-fmk were obtained from R&D Systems, Inc. (Minneapolis, MN, USA). The STAT3 inhibitor S3I-201 was obtained from Calbiochem (San Diego, CA, USA). An EZ-western chemiluminescent detection kit was purchased from Daeil Lab Service Co. (Seoul, Korea).
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