Ethovison xt software

Mice were evaluated for anxiety-related behavior in an elevated zero maze. The maze consists of an annular platform (diameter 46 cm; elevation 50 cm) divided into equally sized quadrants, alternating between open and enclosed (wall height 17 cm). This configuration lacks the ambiguous center region associated with the elevated plus maze^{42 (link)}. The open and closed arms were illuminated to similar levels (30 lx). Individual mice were placed near an enclosed arm of the maze and allowed to freely explore for 5 min. Limelight software (Actimetrics, Wilmette, IL, USA) was used to video record each trial. Ethovison XT software (Noldus, Leesberg, VA, USA) was used to track the position of the mouse, and calculate distance traveled, mean velocity and time spent in closed or open arms (n = 6–14 per strain, genotype, and sex). Trials where mice exited the maze were excluded from analysis. Exclusions by group were as follows: B6.Scn2a^E/+ males – 7 of 13, B6.Scn2a^E/+ females – 5 of 15, F1D2.Scn2a^E/+ males – 2 of 11; all other groups had no exclusions. The proportion of B6.Scn2a^E/+ males excluded due to maze exit was significantly greater than B6.WT males (p=0.0052, Fisher’s exact test), while the proportion of B6.Scn2a^E/+ females excluded was significantly greater than F1D2.Scn2a^E/+ females (p=0.0421, Fisher’s exact test).

Mice were evaluated for anxiety-related behavior in an elevated zero maze at 6 weeks of age. The maze consists of an annular platform (diameter 46 cm; elevation 50 cm) that is divided into equally sized quadrants, alternating between open and enclosed (wall height 17 cm). This configuration lacks the ambiguous center region associated with the elevated plus maze (107 ). Individual mice were placed near an enclosed arm of the maze and allowed to freely explore for 5 min. Limelight software (Actimetrics, Wilmette, IL, USA) was used to video record each trial. Ethovison XT software (Noldus, Leesberg, VA, USA) was used to track the position of the mouse, and calculate distance traveled, mean velocity and time spent in closed or open arms (n = 12–14 per genotype and sex). Trials where mice fell off the maze were excluded from analysis.