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Cortactin antibody 4f11

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The Cortactin antibody 4F11 is a laboratory reagent used in research applications. It is a monoclonal antibody that specifically binds to the Cortactin protein. Cortactin is involved in the regulation of the cytoskeleton and cell motility. The 4F11 clone can be used to detect and study Cortactin in various experimental techniques.

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Close spelling variants of this product

Cortactin (17 citations) Mouse monoclonal anti-cortactin (p80/85) antibody clone 4F11 (2 citations) Mouse antibody against Cortactin (clone 4F11) (2 citations)

2 protocols using cortactin antibody 4f11

1

Cortactin Interactome Profiling in Cells

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SILAC labeled DLD1-wt (light) and DLD1-mt (heavy) cells and SILAC labeled BT20 cells treated with DMSO (light) or J124 (heavy) were lysed with modify RIPA buffer. Equal amount of the protein lysates from DLD1-wt (light) and DLD1-mt (heavy) or BT20 DMSO treated (light) and BT20 J124 treated (heavy) cells were mixed and followed by immunoprecipitation with cortactin antibody 4F11 (05-180, Millipore). The precipitated proteins were subjected to SDS-PAGE and stained with Coomassie brilliant blue R250. The cortactin band was excised for mass spectrometry analysis.
Wu X., Renuse S., Sahasrabuddhe N.A., Zahari M.S., Chaerkady R., Kim M.S., Nirujogi R.S., Mohseni M., Kumar P., Raju R., Zhong J., Yang J., Neiswinger J., Jeong J.S., Newman R., Powers M.A., Somani B.L., Gabrielson E., Sukumar S., Stearns V., Qian J., Zhu H., Vogelstein B., Park B.H, & Pandey A. (2014). Activation of diverse signaling pathways by oncogenic PIK3CA mutations. Nature communications, 5, 4961.
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2

Cortactin Interactome Profiling in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SILAC labeled DLD1-wt (light) and DLD1-mt (heavy) cells and SILAC labeled BT20 cells treated with DMSO (light) or J124 (heavy) were lysed with modify RIPA buffer. Equal amount of the protein lysates from DLD1-wt (light) and DLD1-mt (heavy) or BT20 DMSO treated (light) and BT20 J124 treated (heavy) cells were mixed and followed by immunoprecipitation with cortactin antibody 4F11 (05-180, Millipore). The precipitated proteins were subjected to SDS-PAGE and stained with Coomassie brilliant blue R250. The cortactin band was excised for mass spectrometry analysis.
Wu X., Renuse S., Sahasrabuddhe N.A., Zahari M.S., Chaerkady R., Kim M.S., Nirujogi R.S., Mohseni M., Kumar P., Raju R., Zhong J., Yang J., Neiswinger J., Jeong J.S., Newman R., Powers M.A., Somani B.L., Gabrielson E., Sukumar S., Stearns V., Qian J., Zhu H., Vogelstein B., Park B.H, & Pandey A. (2014). Activation of diverse signaling pathways by oncogenic PIK3CA mutations. Nature communications, 5, 4961.
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