Tac elisa kit

Using the Rat Total antioxidant status (TAC) ELISA Kit (Catalog # MBS1600693, MyBioSource), the total antioxidant status was measured, and all the procedures were performed following the manufacturer’s instructions. The sandwich ELISA kit was utilized for the accurate quantitative detection of the total antioxidant status in the aortic tissue homogenate, and the reaction was terminated by the addition of the acidic stop solution and absorbance was measured at 450 nm.

Determination of total phenolic contents, DPPH radical scavenging activity and FRAP ferric reducing antioxidant power assays were done as described^{9 (link)}. The total phenolic content was estimated using Folin-Ciocalteu method. In brief, 20 µL solution of the plant extract was mixed with 100 µL Folin-Ciocalteu reagent. At ambient temperature, the mixture was incubated for 5 min and then 80 µL of 7.5% Na₂CO₃ solution was added. The mixture was then left for 30 min in the dark and the absorbance was monitored using a microplate reader (Biochrom Asys UVM 340) at 735 nm. The results were presented as gallic acid equivalent (GAE) in mg/g of extract. Total antioxidant capacity (TAC) assay was determined using a commercially available TAC ELISA kit (MBS726896, my biosource, Inc., San Diego, CA, USA) according to the manufacturer’s instructions with ascorbic acid as the reference standard to estimate TAC as described^{10 (link)}.

The antioxidant potential of the extract was evaluated by determining its TAC using a TAC ELISA kit (MBS726896, my BioSource, Inc., San Diego, CA, USA) in accordance with the manufacturer’s instructions and compared to the standard antioxidant, ascorbic acid as previously described Sobeh et al. [3 (link)].