Cells MCF-7 (ATCC number: HTB-22 ) and MDA-MB-231(ATCC number: HTB-26 ) cells were cultured in RPMI-1640 growth medium (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and Eagle’s Minimum Essential Medium (EMEM) (Euroclone SpA, Via Figino, Italy), respectively. Both RPMI-1640 and EMEM medium were supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) 200 mM L-glutamine, and antibiotics; Penicillin-Streptomycin (100 IU/mL–100 µg/mL). Cells were kept in a humidified 5% CO2 at 37 °C. Doxorubicin-resistant MCF-7 (MCF-7/DR) and MDA-MB-231 (MDA-MB-231/DR) cell lines used in this work were previously developed in our lab and maintained using similar conditions of wild-type cell lines [46 (link),47 (link)].
Rpmi 1640 growth medium
Manufactured by Capricorn
Sourced in Germany
RPMI-1640 growth medium is a laboratory cell culture medium used to support the growth and maintenance of a variety of cell types, including immune cells and other mammalian cells. It provides essential nutrients, salts, and other components required for cell proliferation and survival in controlled in vitro conditions.
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Lab products found in correlation
4 protocols using rpmi 1640 growth medium
1
Culturing Breast Cancer Cell Lines
2
Cell Culture Protocols for MCF-7 and MDA-MB-231
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MCF-7 (ATCC number: HTB-22) and MDA-MB-231(ATCC number: HTB-26) cells were cultured in RPMI-1640 growth medium (Capricorn Scientific GmbH, Germany) and Eagle's Minimum Essential Medium (EMEM) (Euroclone SpA, Italy), respectively. Both RPMI-1640 and EMEM media were supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) 200 mM l -glutamine, and antibiotics; Penicillin–Streptomycin (100 IU mL−1-100 μg mL−1). Cells were passaged one time every three days. Accutase was used for cell detachment. Hemocytometer counting with trypan blue staining was used to count viable cells. All experiments were performed for passages range 3 to 10. Cells were maintained in humidified 5% CO2 incubator at 37 °C.
3
Cytotoxicity Evaluation of Zinc Nitrate
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The following chemicals were obtained: zinc nitrate hexahydrate (Zn(NO3)2·6H2O; 98% purity), sodium hydroxide (NaOH; 97% purity), dimethyl sulfoxide (DMSO) of (Sigma-Aldrich, Darmstadt, Germany) analytical grade. An amount of 50 kD MWCO membranes (Spectra/Por7) from REPLIGEN. Corning™ Spin-X™ ultrafiltration devices (0.45 µm). MCF-7 cells (ATCC number: HTB-22) and MDA-MB-231(ATCC number: HTB-26) cells, RPMI-1640 growth medium (Capricorn Scientific GmbH, Hessen, Germany). Eagle’s Minimum Essential Medium (EMEM) (Euroclone SpA, Pavia, Italy). An amount of 10% (v/v) fetal bovine serum (FBS), L-glutamine, penicillin-streptomycin antibiotics and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) from Thermo Fisher Scientific (Waltham, MA, USA).
4
Cell Culture of Cancer Cell Lines
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MCF-7 (ATCC number: HTB-22) and A549 (ATCC Number: CCL-185) cell lines were cultured in RPMI-1640 growth medium (Capricorn Scientific GmbH, Germany), MDA-MB-231(ATCC number: HTB-26) and human skin cells (fibroblasts) (ATCC® PCS-201-012) cell lines were cultured Eagle's Minimum Essential Medium (EMEM) (Euroclone SpA, Italy). Both RPMI-1640 and EMEM medium were supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) 200 mM l -glutamine, and antibiotics, penicillin–streptomycin (100 IU/mL-100 μg mL−1). Cells were maintained in humidified 5% CO2 incubator at 37 °C.
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