After fixation in Bouins solution, one testis/mouse was cut in half horizontally and embedded in paraffin. Testis sections (8 µm) were stained with periodic acid-Schiff reagent and counterstained with hematoxylin to facilitate analysis and staging of spermatids based on acrosomal morphology (Russell et al. 1990 ). Composite images showing a complete transverse section from each testis (20× magnification) were generated using an Olympus BX51 microscope equipped with an Olympus DP72 digital camera, a motorized stage and MetaMorph automation & image analysis software (Molecular Devices, Sunnyvale, CA). MetaMorph measurements of the height, width, perimeter, and area of each section also were recorded. Each composite image was examined in detail to determine the number of seminiferous tubule cross sections and the number of tubules exhibiting defects, including vacuoles, abnormal or degenerating germ cells, immature germ cells in the lumen, and significant germ cell loss.
Metamorph automation image analysis software
Manufactured by Molecular Devices
Sourced in United States
MetaMorph is an automation and image analysis software for life science research. It provides tools for controlling microscope hardware, acquiring and processing digital images, and analyzing image data.
Automatically generated - may contain errors
2 protocols using metamorph automation image analysis software
1
Testicular Histology Analysis Protocol
2
Quantifying Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Random migration was monitored by time-lapse microscopy and analysed using MetaMorph Automation & Image Analysis Software (Molecular Devices, USA); 30,000 cells were plated in matrigel-coated 6 well plates and stimulated with 10% serum. Transwell migration assays were performed using 24-well cell culture inserts (BD Biosciences, USA) as described (77 (link)). Invasion index was obtained as the percentage of transwell migration with/without coating of the inserts with 50μL 5% growth factor-reduced (GFR) matrigel (BD Biosciences, USA). For colony outgrowth assays, 2000 cells were plated into 50μL GFR matrigel in 96-well plates and incubated for 7 days.
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