Anti lkb1

Manufactured by Santa Cruz Biotechnology

Anti-LKB1 is a laboratory product offered by Santa Cruz Biotechnology. It is an antibody that specifically targets the LKB1 protein. LKB1 is a serine/threonine kinase that plays a critical role in cellular energy homeostasis and acts as a tumor suppressor. The core function of Anti-LKB1 is to facilitate the detection and study of the LKB1 protein in various experimental applications.

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Lab products found in correlation

Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti β catenin, by BD (1 mentions) Anti hsf1, by Cell Signaling Technology (1 mentions) Phosphorylated akt s473, by Cell Signaling Technology (1 mentions) Rabbit anti akt antibody, by Cell Signaling Technology (1 mentions) Nlrp3, by Cell Signaling Technology (1 mentions) Hrp conjugated rabbit anti mouse igg, by Thermo Fisher Scientific (1 mentions) Phosphorylated ampkα, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Mouse monoclonal anti-p-LKB1 (2 citations)

2 protocols using anti lkb1

Immunohistochemistry for Key Signaling Proteins

Cited 1 time

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Immunohistochemistry was performed as described previously.^{36 (link)} Primary antibodies were used including anti-β-catenin (1:1000, BD,
610154), anti-HSF1 (1:200, Cell Signaling Technology, No. 12972), anti-LKB1
(1:100, Santa Cruz, sc-32245), and anti-PCNA (1:1000, Santa Cruz, sc-7907).

Wang S., Ma K., Zhou C., Wang Y., Hu G., Chen L., Li Z., Hu C., Xu Q., Zhu H., Liu M, & Xu N. (2019). LKB1 and YAP phosphorylation play important roles in Celastrol-induced β-catenin degradation in colorectal cancer. Therapeutic Advances in Medical Oncology, 11, 1758835919843736.

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Western Blot Analysis of Phosphorylated Signaling Proteins

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Proteins in the cell lysate were separated by SDS-PAGE on a 10% polyacrylamide gel and transferred to polyvinylidene fluoride membranes (GE or ATTO). After the membranes were blocked with 1% BSA in tris buffered saline with tween 20 they were incubated with the following primary antibodies against phosphorylated AMPKα (T172; Cell Signaling Technology), phosphorylated Akt (S473; Cell Signaling Technology), NLRP3 (Cell Signaling Technology), and phosphorylated LKB1 (liver kinase B1; S431; Santa Cruz Biotechnology). The secondary antibody was HRP-conjugated goat anti-rabbit IgG (Cappel) and HRP-conjugated rabbit anti-mouse IgG (Invitrogen). Antibodies were detected using Chemi-Lumi One Ultra (Nakarai). Membranes were stripped in buffer (100 mmol/L 2-mercaptoethanol, 2% SDS, and 62.5 mmol/L Tris-HCl, pH 6.7), blocked, reincubated with anti-AMPKα rabbit antibody (Cell Signaling Technology), anti-AKT rabbit antibody (Cell Signaling Technology), anti-LKB1 (Santa Cruz Biotechnology), anti-α-tubulin rabbit antibody (Proteintech), or anti-β-actin rabbit antibody (Proteintech) and detected as described above.

Kimura Y., Yanagida T., Onda A., Tsukui D., Hosoyamada M, & Kono H. (2020). Soluble Uric Acid Promotes Atherosclerosis via AMPK (AMP-Activated Protein Kinase)-Mediated Inflammation. Arteriosclerosis, thrombosis, and vascular biology, 40(3).

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