Anti human b7 h3

The anti-human B7-H3, MMP2 and MMP9 antibodies used for immunohistochemistry were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). The anti-human B7-H3, E-Cadherin, N-Cadherin, Vimentin, β-catenin, CD44, Oct4, Akt, phosphorylated Akt, Smad1 and Smad9 antibodies were from Abcam (Cambridge, MA, United States). The anti-human phosphorylated Smad1/5 and Smad1/5/9 antibodies were from Cell Signaling Technology, Inc. (Danvers, MA, United States). The anti-human CD133 antibody for flow cytometry was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The horseradish peroxidase-conjugated secondary anti-mouse and anti-rabbit antibodies and the GAPDH antibody were from Beyotime (Nantong, China). The fluorescent dye DiI that was used to label the cell membrane was from Keygentec (Nanjing, China). The Jak2-specific inhibitor AG490 was purchased from Sigma-Aldrich (St. Louis, MO, United States). The PI3K-specific inhibitor LY294002 was from Selleckchem (Houston, TX, United States). The Matrigel^TM matrix used in the invasion assay was from BD (San Jose, CA, United States).

Expression of cell surface antigen was detected by flow cytometry. B7H3 was stained with Anti-Human B7H3 (Abcam, UK), CCR7 was stained with Anti-Human CCR7 (Abcam, UK), CD45RO was stained with Anti-Human CD45RO (Abcam, UK), CD44 was stained with Anti-Human CD44 (Abcam, UK), CD133 was stained with Anti-Human CD133 (Abcam, UK).