Anti ppkc

Manufactured by Abcam

Sourced in United Kingdom

Anti-pPKC is a laboratory reagent used to detect and quantify the phosphorylated form of protein kinase C (PKC) in various biological samples. It is a highly specific antibody that binds to the phosphorylated epitope of PKC, enabling researchers to study the activation and regulation of this important signaling molecule.

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2 protocols using anti ppkc

Multiplex Immunoblotting Antibody Validation

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All chemicals were obtained from Sigma-Aldrich unless otherwise specified. The following antibodies were used in this study: rabbit anti-UCP1 (Abcam, ab155117, GR3233606-10 1:2000), anti-Parvalbumin (ABclonal, A13538, Lot0054370201 1:1000), anti-Transferrin (Abbkine, ABM40235, 1:1000), anti-pSTST6 (Cell Signaling Tech, 56554, 1:2000), anti-STAT6 (Cell Signaling Tech, 9362, 1:2000), anti-pAKT (Ser473) (Cell Signaling Tech, 4060, 1:2000), anti-pAKT (Thr308) (Cell Signaling Tech, 13038,1:2000), anti-AKT (Cell Signaling Tech, 2920, 1:2000), anti-GAPDH (Santa Cruz, sc32233, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-pERK (Cell Signaling Tech, 4370, 1:2000), anti-ERK (Cell Signaling Tech, 4695, 1:2000), anti-pPKC (Abcam, ab180848, 1:2000), anti-PKC (Abcam, ab179522, 1:2000), anti-p4EBP1 (Cell Signaling Tech, 2855, 1:2000), anti-4EBP1 (Cell Signaling Tech, 9452, 1:2000), anti-ACTB (Sigma Aldrich, A3854, 1:10000), anti-Flag (Sigma-Aldrich, F7425, 1:10000), HRP-conjugated goat anti-Rabbit IgG (Cell Signaling Tech, 7074, 1:10000), anti-pGSK-3β (Cell Signaling Tech, 5558, 1:2000), anti-GSK-3β (Cell Signaling Tech, 12456, 1:2000). Rictor antibody (Abcam, ab70374, 1:100), mTOR antibody (Cell Signaling Tech, 2983, 1:100) (Supplementary Table 2).

Lin S., Zhang A., Yuan L., Wang Y., Zhang C., Jiang J., Xu H., Yuan H., Yao H., Zhang Q., Zhang Y., Lou M., Wang P., Zhang Z.N, & Luan B. (2022). Targeting parvalbumin promotes M2 macrophage polarization and energy expenditure in mice. Nature Communications, 13, 3301.

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Molecular Mechanisms in Inflammatory Response

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Trypsin-EDTA and Dulbecco's modified Eagle's medium (DMEM) were bought from Gibco (Grand Island, NY, USA). PD98059, dihydroethidium (DHE), Apocynin, Diphenyleneiodonium (DPI), Polyinosinic-polycytidylic acid (poly(I:C)), collagenase B, Pyrrolidine dithiocarbamate (PDTC), penicillin and streptomycin were all purchased from Sigma (St. Louis, MO, USA). Anti-PKR, anti-p-PKR,anti-PKC, anti-p-PKC, anti-NOX-1, anti-p47, anti-Rac-1, were all obtained from Abcam (Cambridge, UK). Anti-β-actin, anti-ERK, anti-p-ERK, anti-COX-2, anti-PPAR-γ were all obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated anti-rabbit secondary antibodies were purchased from Transduction Laboratories (CA, USA). Antioxidant enzymes kits were obtained from EMD Millipore (Calbiochem, Gibbstown, NJ). IL-8 ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA).

Ma C.H., Wu C.H., Jou I.M., Tu Y.K., Hung C.H., Hsieh P.L, & Tsai K.L. (2017). PKR activation causes inflammation and MMP-13 secretion in human degenerated articular chondrocytes. Redox Biology, 14, 72-81.

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