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Anti ppkc

Manufactured by Abcam
Sourced in United Kingdom

Anti-pPKC is a laboratory reagent used to detect and quantify the phosphorylated form of protein kinase C (PKC) in various biological samples. It is a highly specific antibody that binds to the phosphorylated epitope of PKC, enabling researchers to study the activation and regulation of this important signaling molecule.

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2 protocols using anti ppkc

1

Multiplex Immunoblotting Antibody Validation

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All chemicals were obtained from Sigma-Aldrich unless otherwise specified. The following antibodies were used in this study: rabbit anti-UCP1 (Abcam, ab155117, GR3233606-10 1:2000), anti-Parvalbumin (ABclonal, A13538, Lot0054370201 1:1000), anti-Transferrin (Abbkine, ABM40235, 1:1000), anti-pSTST6 (Cell Signaling Tech, 56554, 1:2000), anti-STAT6 (Cell Signaling Tech, 9362, 1:2000), anti-pAKT (Ser473) (Cell Signaling Tech, 4060, 1:2000), anti-pAKT (Thr308) (Cell Signaling Tech, 13038,1:2000), anti-AKT (Cell Signaling Tech, 2920, 1:2000), anti-GAPDH (Santa Cruz, sc32233, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-pERK (Cell Signaling Tech, 4370, 1:2000), anti-ERK (Cell Signaling Tech, 4695, 1:2000), anti-pPKC (Abcam, ab180848, 1:2000), anti-PKC (Abcam, ab179522, 1:2000), anti-p4EBP1 (Cell Signaling Tech, 2855, 1:2000), anti-4EBP1 (Cell Signaling Tech, 9452, 1:2000), anti-ACTB (Sigma Aldrich, A3854, 1:10000), anti-Flag (Sigma-Aldrich, F7425, 1:10000), HRP-conjugated goat anti-Rabbit IgG (Cell Signaling Tech, 7074, 1:10000), anti-pGSK-3β (Cell Signaling Tech, 5558, 1:2000), anti-GSK-3β (Cell Signaling Tech, 12456, 1:2000). Rictor antibody (Abcam, ab70374, 1:100), mTOR antibody (Cell Signaling Tech, 2983, 1:100) (Supplementary Table 2).
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2

Molecular Mechanisms in Inflammatory Response

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Trypsin-EDTA and Dulbecco's modified Eagle's medium (DMEM) were bought from Gibco (Grand Island, NY, USA). PD98059, dihydroethidium (DHE), Apocynin, Diphenyleneiodonium (DPI), Polyinosinic-polycytidylic acid (poly(I:C)), collagenase B, Pyrrolidine dithiocarbamate (PDTC), penicillin and streptomycin were all purchased from Sigma (St. Louis, MO, USA). Anti-PKR, anti-p-PKR,anti-PKC, anti-p-PKC, anti-NOX-1, anti-p47, anti-Rac-1, were all obtained from Abcam (Cambridge, UK). Anti-β-actin, anti-ERK, anti-p-ERK, anti-COX-2, anti-PPAR-γ were all obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated anti-rabbit secondary antibodies were purchased from Transduction Laboratories (CA, USA). Antioxidant enzymes kits were obtained from EMD Millipore (Calbiochem, Gibbstown, NJ). IL-8 ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA).
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