The cultured cells were characterized based on their mesenchymal origin by immunostaining for vimentin. Once fixed in acetone, they were stained with mouse anti-vimentin (Santa Cruz Biotechnology, Dallas, TX, USA) followed by DyLight 594 conjugated anti-mouse IgG (Vector Labs, Burlingame, CA, USA). The slides were mounted with a mounting medium containing DAPI (4’,6-diamidino-2-phenylindole dihydrochloride hydrate) to stain the nucleus. The images of these cells were captured by a fluorescence microscope (Leica TCS-SPE; Leica, Wetzlar, Hesse, Germany) 21 .
Dylight 594 conjugated anti mouse igg
Manufactured by Vector Laboratories
Sourced in United States
DyLight 594 conjugated anti-mouse IgG is a secondary antibody reagent. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various applications such as western blotting, immunohistochemistry, and flow cytometry.
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Lab products found in correlation
2 protocols using dylight 594 conjugated anti mouse igg
1
Characterizing Mesenchymal Cells by Vimentin
2
Vimentin Characterization of Cultured Cells
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Cultured cells were characterized on the basis of their mesenchymal origin by positivity for vimentin. Cells were fixed in acetone and immunostaining substance with mouse anti-vimentin (Santa Cruz Biotechnology, Dallas, TX, USA) followed by DyLight 594 conjugated anti-mouse IgG (Vector Labs, Burlingame, CA, USA). Slides were then mounted using mounting medium containing DAPI (4’,6-diamidino-2-phenylindole dihydrochloride hydrate) for DNA staining. Images were captured by an inverted confocal microscope (Leica TCS-SPE; Leica, Wetzlar, Hesse, Germany).
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