761 compact ion chromatograph

Manufactured by Metrohm

Sourced in Switzerland

The 761 Compact Ion Chromatograph is a laboratory instrument designed for the analysis of ions in liquid samples. It is capable of separating and quantifying various ionic species, such as anions, cations, and organic acids, using ion chromatography technology.

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Lab products found in correlation

Flash ea 1112, by Thermo Fisher Scientific (1 mentions) Nanocolor cod 1500, by Macherey-Nagel (1 mentions) Gc 2014 gas chromatograph, by Shimadzu (1 mentions) 930 compact ic flex, by Metrohm (1 mentions) Milli q water, by Merck Group (1 mentions)

7 protocols using 761 compact ion chromatograph

Lipidated Peptide C16KTTβAH Characterization

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The lipidated peptide C₁₆KTTβAH was purchased
from CS Bio (Menlo Park, California) and supplied as the trifluoroacetate
(TFA) salt. Purity was 98.19% by analytical high-performance liquid
chromatography (HPLC) using acetonitrile (0.1% TFA)/water (0.1% TFA)
gradient. The molar mass measured by electrospray ionization (ESI)–mass
spectrometry (MS) was 795 g mol^–1 (795.02 g mol^–1 expected). Elemental analysis of CHN and O was performed
by combustion and pyrolysis using a Thermo FlashEA 1112 instrument
and F analysis by ion chromatography using a Metrohm 761 Compact Ion
Chromatograph following oxygen flask combustion of the sample. These
measurements provided C 50.78%, H 5.18%, N 11.64%, O 21.09%, and F
2.96%, consistent with the presence of TFA salt. Scheme S1 shows the chemical structure of C₁₆KTTβAH
and Figure S1 contains the HPLC and ESI–MS
spectra. Solutions of peptides were prepared by mixing weighed amounts
of water and peptide or phosphate-buffered saline (PBS) and peptide.
The native pH of the lipopeptide in water was found to be 6. The mixtures
were left to equilibrate for 24 h before characterization. The solvent
used to prepare the solution is only indicated for samples dissolved
in PBS.

Castelletto V., Edwards-Gayle C.J., Greco F., Hamley I.W., Seitsonen J, & Ruokolainen J. (2019). Self-Assembly, Tunable Hydrogel Properties, and Selective Anti-Cancer Activity of a Carnosine-Derived Lipidated Peptide. ACS Applied Materials & Interfaces, 11(37), 33573-33580.

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Quantifying Organic Acid Production

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Organic acids in the samples were measured with 761 Compact Ion Chromatograph (Metrohm, Switzerland) with a Metrosep Organic acids 250/7.8 column and a Metrosep Organic acids Guard/4.6 guard column. The eluent consists of 1 mM H₂SO₄ at a flow rate of 0.8 mL/min. The production of organic acids was calculated as the concentration detected in the liquid that the biofilm was grown normalized for the organic acid concentrations detected in sterile BHI 2 medium.

Chatzigiannidou I., Teughels W., Van de Wiele T, & Boon N. (2020). Oral biofilms exposure to chlorhexidine results in altered microbial composition and metabolic profile. NPJ Biofilms and Microbiomes, 6, 13.

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Comprehensive Wastewater Characterization Methods

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Total solids (TS), total suspended solids (TSS), volatile suspended solids (VSS), volatile solids (VS), Kjeldahl nitrogen (TKN) and total COD were measured according to Standard Methods (Greenberg et al. 1992) . Soluble COD was measured using Nanocolor COD1500 or 15000 test kits (Machery-Nagel, Düren, Germany), according to the manufacturer's instructions. The concentrations of NH4 + , Na + and K + were measured on a 761 Compact Ion Chromatograph (Metrohm, Herisau, Switzerland), which was equipped with a Metrosep C6-250/4.0 main column, a Metrosep C4 Guard/4.0 guard column and a conductivity detector. The eluent contained 1.7 mM HNO3 and 1.7 mM dipicolinic acid. Samples were centrifuged at 3000g for 3 min with a Labofuge 400 Heraeus centrifuge (Thermo Fisher Scientific Inc, Merelbeke, Belgium), filtered over a 0.22 µm filter (type PA-45/25, Macherey-Nagel, Germany) and diluted with Milli-Q water to reach the desired concentration range for quantification between 1 and 100 mg L -1 . The pH was measured with a C532 pH meter, and conductivity was determined with a C833 conductivity meter (Consort, Turnhout, Belgium). The biogas composition was measured using a Compact Gas Chromatograph (Global Analyser Solutions, Breda, The Netherlands) (S5). The different VFA (C2-C8) were measured with a GC-2014 Gas Chromatograph (Shimadzu®, The Netherlands) (S6).

Law C.K.Y., De Henau R, & De Vrieze J. (2020). Feedstock thermal pretreatment selectively steers process stability during the anaerobic digestion of waste activated sludge. Applied microbiology and biotechnology, 104(8).

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Organic Acid Analysis of Biofilm Supernatant

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Concentrations of organic acids in the filter sterilized supernatant of the multi-species biofilms were measured using a 761 Compact Ion Chromatograph (Metrohm, Switzerland) with a Metrosep Organic acids Guard/4.6 guard column. The eluent consisted of 1 mM H₂SO₄ at a flow rate of 0.8 mL min⁻¹. Organic acid production/consumption was calculated as the organic acid concentrations detected in the filter sterilized biofilm supernatant, minus the concentrations of those organic acids detected in sterile modified BHI medium.

Verspecht T., Van Holm W., Boon N., Bernaerts K., Daep C.A., Masters J.G., Zayed N., Quirynen M, & Teughels W. (2021). Potential prebiotic substrates modulate composition, metabolism, virulence and inflammatory potential of an in vitro multi-species oral biofilm. Journal of Oral Microbiology, 13(1), 1910462.

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Metabolite Profiling of Colon Samples

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The colon compartments were additionally sampled the first day after inoculation (before initiating semi-continuous flow-through), after 4.5 transits (halfway stabilisation phase) and after 9 distal colon transits (stabilised community) to analyse ammonium and lactate concentrations.
Samples for ammonium and lactate analyses were filtered through a 0.22μm filter and diluted with Milli-Q water (Merck Millipore, Overijse, Belgium). Ammonium samples were diluted 20 times and analysed with a 761 Compact Ion Chromatograph (Metrohm, Switzerland) equipped with a conductivity detector. Lactate samples were diluted 5 times and measured with a 930 Compact IC Flex (Metrohm, Switzerland) with inline bicarbonate removal and a conductivity detector [28 (link)].

Minnebo Y., Delbaere K., Goethals V., Raes J., Van de Wiele T, & De Paepe K. (2023). Gut microbiota response to in vitro transit time variation is mediated by microbial growth rates, nutrient use efficiency and adaptation to in vivo transit time. Microbiome, 11, 240.

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Ion Chromatography and Water Analysis

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Nitrite, nitrate and sulfate were determined on a 761 Compact Ion Chromatograph (Metrohm, Switzerland). Ammonium nitrogen (via steam distillation) and total suspended solids (TSS) and volatile suspended solids (VSS) were analyzed according to Standard methods (APHA, 1992) . The pH was measured with a Consort C5010 meter equipped with a Consort pH electrode (Consort, Belgium) and conductivity with a Consort C6010 meter with a Metrohm conductivity probe (Metrohm, Switzerland).

Vandekerckhove T.G.L., Kobayashi K., Janda J., Van Nevel S, & Vlaeminck S.E. (2018). Sulfur-based denitrification treating regeneration water from ion exchange at high performance and low cost. Bioresource technology, 257.

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Measurement of Water Quality Parameters

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NH 4 + , TKN, TSS, VSS and biochemical oxygen demand (BOD 5 ) were measured according to standard methods (APHA, 1998) . COD was measured by photometric methods using Nanocolor test tubes (Machereye-Nagel, Germany). Nitrite and nitrate were determined on a 761 Compact Ion Chromatograph (Metrohm, Switzerland) equipped with a conductivity detector.

Peng L., Carvajal-Arroyo J.M., Seuntjens D., Prat D., Colica G., Pintucci C, & Vlaeminck S.E. (2017). Smart operation of nitritation/denitritation virtually abolishes nitrous oxide emission during treatment of co-digested pig slurry centrate. Water research, 127.

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