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Bioflash

Manufactured by Werfen
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Sourced in Spain

Bioflash is a compact, automated chemiluminescence immunoassay analyzer designed for the quantitative determination of various analytes in clinical laboratory settings. It offers efficient sample processing and reliable results.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (2 mentions) C8000, by Roche (1 mentions) Turbidimetry system, by Abbott (1 mentions) Combur test, by Roche (1 mentions) Nephelometry system, by Beckman Coulter (1 mentions) Sta immunodef 8, by Stago (1 mentions) Sta r max analyzer, by Stago (1 mentions)

5 protocols using bioflash

1

Vasculitis Biomarker Detection Protocol

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Blood samples were collected from patients with symptoms compatible with vasculitis. Blood was centrifuged for 5 min at 3000 rpm to separate the serum, and anti-MPO and anti-PR3 were determined by chemiluminescence (Bioflash, Werfen). The measuring range for anti-MPO antibodies was 3.2–739.8 CU and for anti-PR3 antibodies was 2.3–3285.3 CU. ANCA were not tested by indirect immunofluorescence (IIF).
Statistical analysis was performed using Graph Pad Prism software 6.0 version. Cut-off points were established following a receiver operating characteristic (ROC) curve. The results were expressed as mean, standard deviation (SD), and percentages. The distribution of continuous variables was assessed using Kolmogorov–Smirnov/Shapiro–Wilk tests. Comparisons were based on non-parametric U-Mann–Whitney tests. A two-sided p-value < 0.05 was considered statistically significant.
Renuncio-García M., Calvo-Río V., Benavides-Villanueva F., Al Fazazi S., Rodríguez-Vidriales M., Escagedo-Cagigas C., Martín-Penagos L., Irure-Ventura J., López-Hoyos M, & Blanco R. (2023). ANCA detection with solid phase chemiluminescence assay: diagnostic and severity association in vasculitis. Immunologic Research, 72(1), 128-133.
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2

Autoantibody Measurement Protocol

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Rheumatoid factor were measured by immunonephelometry (BN2 siemens); anti-citrullinated protein antibody by chemiluminescence (Bioflash, Werfen), and C-reactive protein by immunoturbidimetry (C8000 roche).
Marouen S., du Cailar G., Audo R., Lukas C., Vial G., Tournadre A., Barrat E., Ribstein J., Combe B., Morel J, & Daien C.I. (2017). Sodium excretion is higher in patients with rheumatoid arthritis than in matched controls. PLoS ONE, 12(10), e0186157.
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3

Fecal Calprotectin Quantification Protocol

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Fecal calprotectin was measured using the BIOFLASH (Werfen, Barcelona, Spain) chemo-luminescent analyzer platform according to ISO 15,189 standards. Assay sensitivity was greater than 20 µg/mL. The total protein concentration of the samples was measured by the colorimetric method (BCA Protein assay, Thermo Fisher Scientific).
Sereme Y., Michel M., Mezouar S., Guindo C.O., Kaba L., Grine G., Mura T., Mège J.L., Tran T.A., Corbeau P., Filleron A, & Vitte J. (2022). A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases. Journal of Clinical Medicine, 11(10), 2749.
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4

Autoimmune Antibody Profiling Protocol

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Anti-dsDNA, Sm, SSB-La, SSA-Ro and U1-RNP levels were determined in serum using BIO-FLASH® chemiluminescence (Werfen, Barcelona, Spain). Anti-C1q levels in serum were quantified by Quanta Lite Anti-C1q ELISA (Inova Diagnostic, San Diego, CA, USA). Anti-histone presence in serum was established using Euroline ANA Perfil (Euroimmune, Lübeck, Germany). Levels of C3 in serum were determined using the Nephelometry System (Beckman Coulter Diagnostics, Nyon, Switzerland). Albumin/creatinine ratio was determined in serum by the turbidimetry system (Abbott, Chicago, IL, USA). The presence of hematuria was determined by the Combur test (Roche, Basilea, Switzerland). Anti-dsDNA antibodies were considered positive when levels were >35 UI/mL. Sm, SSB-La, SSA-Ro and U1-RNP antibodies were considered positive when levels were >20 Chemoluminiscence Units and C1q when levels were >20 U/mL according to manufacturer’s study. C3 levels were considered decreased when they were below 85 mg/dL according to manufacturer’s study. Microalbuminuria was considered when albumin/creatinine ratio was >2.5 mg/mmol.
Mariscal A., Zamora C., Magallares B., Salman-Monte T.C., Ortiz M.À., Díaz-Torné C., Castellví I., Corominas H, & Vidal S. (2021). Phenotypic and Functional Consequences of PLT Binding to Monocytes and Its Association with Clinical Features in SLE. International Journal of Molecular Sciences, 22(9), 4719.
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5

Plasma VWF and FVIII Measurement Protocol

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Plasma samples from Leiden were measured for VWF:Ag and VWF and FVIII activity by the clinical chemistry laboratory at the LUMC, Leiden. VWF:Ag in plasma was determined using the STA LIA VWF:Ag test (Stago) and was analyzed on the Sta‐R Max analyser (Stago) with a commercial STA VWF:Ag calibrator (STA Unicalibrator, Stago) as reference. VWF activity was determined with the VWF ristocetin‐triggered GPIb binding assay (VWF:GPIbR) with HemosIL AcuStar VWF:RCo reagent (Werfen IL). Samples were analyzed on the BIO‐FLASH (Werfen) and a commercial calibrator (supplied with the HemosIL AcuStar VWF:RCo) was used as reference. FVIII activity was determined using an automated one‐stage clotting assay on the STA‐R MAX analyzer (Stago) with Sta‐immunodef VIII (Stago) and STA‐CK Prest 5 (APTT; Stago) reagents. Commercial normal pool plasma (STA Unicalibrator, Stago) was used as reference. At Kingston, only VWF:Ag was measured in the plasma samples, and this was done by ELISA as previously described.22
de Boer S., Bowman M., Notley C., Mo A., Lima P., de Jong A., Dirven R., Weijers E., Lillicrap D., James P, & Eikenboom J. (2020). Endothelial characteristics in healthy endothelial colony forming cells; generating a robust and valid ex vivo model for vascular disease. Journal of Thrombosis and Haemostasis, 18(10), 2721-2731.
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